Nucleic Acid Labeling

DIG DNA Labeling and Detection

Digoxigenin (DIG) labeling technology is an ideal alternative to hazardous radioactive isotopes for nucleic acid labeling. Flexibly use colorimetric, luminescent or fluorescent signal detection and achieve high sensitivity with low background in very short exposure times. Use robust procedures and established protocols for low background and a high signal-to-noise ratio. As exclusive distributors of the Roche DIG System, we are committed to providing researchers with the high specificity and sensitivity that they require to detect nucleic acids and enable their next breakthrough. The DIG System offers several benefits:

• Specificity: DIG antibodies do not bind to other substrates.
• Versatility: Use DIG labeled probes for filters and in situ hybridization.
• Proven: Thousands of publications show why DIG is superior to radioactivity.
• Safety: Label and detect without the dangers or inconvenience of radioactivity.

For additional information on DIG protocols, reagents, and resources, please visit our DIG Labeling Methods resource page.

Digoxigenin (DIG) Labeling Methods

Originally based on a steroid isolated from digitalis plants, the only known source of digoxigenin, the DIG system is a powerful tool for nucleic hybridization analysis. Provided the limited natural occurrence of this molecule in nature makes this an ideal labeling molecule as the anti-DIG antibody does not bind to other biological molecules, ensuring high specificity to the target. Once the stabilized nucleic acid hybridizes with the DIG-labeled probe, anti-digoxigenin antibodies coupled with either horseradish peroxidase (HRP) or alkaline phosphatase, rhodamine or fluorescein are used for subsequent chemiluminescent or fluorescent detection.