Isolation and purification of proteins requires disruption of cells or tissue samples and extraction of the relevant protein fraction. This step is critical as processing methods can affect the target protein’s integrity and activity, or otherwise expose it to degradative conditions. A variety of reagents and enzymes for cell lysis and protein extraction provide an array of options tailored for specific cell types and application needs.
Radioimmunoprecipitation assay (RIPA) buffer is used for rapid cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. RIPA buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with immunoreactivity and biological activity. RIPA buffer also minimizes non-specific protein binding interactions to ensure low background in immunoprecipitation and molecular pull-down assays.
BugBuster® protein extraction reagents are optimized for protein extraction from E. coli under non-denaturing conditions.
BugBuster® Master Mix combines the BugBuster® bacterial protein extraction reagent with Benzonase® nuclease and rLysozyme™ solution for convenient, all-in-one protein extraction and nucleic acid digestion.
CelLytic™ lysis reagents are specifically formulated to lyse and extract cellular proteins under non-denaturing conditions based on the cell or tissue type and target protein localization.
PopCulture® reagents enable protein extraction from cells directly in the culture medium with no centrifugation required. Insect PopCulture® reagent lyses insect cells directly in serum-free medium and is ideal for expression screening of many small samples.