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Product Information Sheet - ROANNV
11Annexin-V-FLUOS Staining Kit
sigma-aldrich.com 5
2.2. Protocols
Staining of cell suspensions
Wash 1 × 106 cells with PBS and centrifuge cells at 200 × g for 5 minutes.
Product Information Sheet-coul-ro
682 001
200 ml, settled resin volume 05 893 801 001
cOmplete His-Tag Purification
Column
1 column, 1 column containing
5 ml of cOmplete His-Tag Purification Resin
06 781 535 001
5 x 1 ml
Data Sheet - I8034
571 (1988).
3. Namen, A. E., et al., J. Exp. Med., 167, 988 (1988).
4. Hofmeister, R., et al., Cytokine Growth Factor Rev.,
10, 41-60 (1999).
5. Goodwin, R.G., et al., Cell, 60, 941-951 (1990).
BKR
User Guide-RAB0184
12 µL 300 µL 300 µL 300 µL 300 µL 300 µL
Std 1 Std 2 Std 3 Std 4 Std 5 Std 6 0 Std
Diluent
volume
Std +
400 µL
988 µL 300 µL 300 µL
Product Information Sheet - ROAMYC
Affinity (3F10)
25 �g 11 988 506 001
Anti-HA-Peroxidase, High
Affinity (3F10)
25 �g 12 013 819 001
Anti-HA Affinity Matrix 1 ml 11 815 016 001
HA Peptide 5 mg 11 666 975 001
Data Sheet - 30020
antibiotic produced by certain
strains of Streptomyces orchidaceus or S. garphalus.1-5
D-cycloserine (at 100 to 200 µg/ml) inhibits the synthesis of bacterial cell walls (involving
peptidoglycan synthesis
Production and purification of virus-like particle (VLP) based Hepatitis C vaccine candidate
Polygard® CN filters of nominal pore sizes of 10, 5, 0.6 and 0.3 μm (all
pleated polypropylene depth filters) were evaluated, all operated at inlet flux of 988 LMH.
Ultrafiltration/Diafiltration: Pellicon
Enhanced Sensitivity Immunogenicity (ADA) Assay - Next Generation SMC™ Technology
Matri
0.00 68 39 22 2
0.05 72 37 20 80
0.15 68 35 22 162
0.46 68 47 23 357
1.37 92 49 25 988
4.12 110 60 34 2090
12.35 206 112 55 2946
37.04 491 230 125 5892
111.11 897 647 265 8293
333.33 2318
Product Information Sheet-dpni-ro
in diagnostic procedures.
y Version: 20
Content Version: March 2020
Cat. No. 10 742 988 001 1,000 U
10 U/μl
Store product at −15 to −25°C.
Product Information Sheet-10988537001
deoxy-guanosine-5’-
triphosphate
For life science research only.
Not for use in diagnostic procedures.
y Version: 08
Content Version: June 2021
Lithium salt
Cat. No. 10 988 537 001 2 μmol
200 μl
Product Information Sheet-roaha
, kits
Anti-HA-Fluorescein, High Affinity 25 μg 11 988 506 001
PVDF Western Blotting Membranes 1 roll, 30 cm x 3.00 m 03 010 040 001
Tween 20 50 ml, 5 x 10 ml 11 332 465 001
Western Blocking Reagent, Solution
Protein Phosphatases Technical Bulletin
activity. The kit includes human recombinant
PTP1B enzyme (amino acid residues 1 - 322), the EGFR (988 - 998) phos-
phopeptide substrate (Km = 3.9 mM), the PTP1B inhibitor RK-682, phos-
phate standard
Metal Chelate Affinity Chromatography
Morganti L. (2003) Current and prospective applications of metal ion–protein binding; J.Chromatogr. A 988, 1-23
• Mokrzycki-Issartel N., Bouchon B., Farrer S., Berland P., Laparra H., Madelmont J-C. & Theisen
Product Information Sheet - ROAHA
Affinity (3F10)
25 �g 11 988 506 001
Anti-HA-Peroxidase, High
Affinity (3F10)
25 �g 12 013 819 001
Anti-HA Affinity Matrix 1 ml 11 815 016 001
HA Peptide 5 mg 11 666 975 001
Product Information Sheet - 11585878001
Reagent 0.4 ml
1 ml
5 × 1 ml
11 815 091 001
11 814 443 001
11 988 387 001
FuGENE® HD Transfection Reagent 0.4 ml (up to 120 transfections)
1 ml (up to 300 transfections)
5
Product Information Sheet - 11666851001
Affinity (3F10)
25 �g 11 988 506 001
Anti-HA-Peroxidase, High
Affinity (3F10)
25 �g 12 013 819 001
Anti-HA Affinity Matrix 1 ml 11 815 016 001
HA Peptide 5 mg 11 666 975 001
Product Information Sheet - 11539426001
Transfection Reagent 1 ml
(300 transfections)
0.4 ml
(120 transfections)
5 × 1 ml multipack
11 814 443 001
11 815 091 001
11 988 387 001
Anti-CAT-coated Microplates, transparent 192 tests 11 465
Product Information Sheet - I1408
et al., Current and Prospective
Applications of Metal Ion-Protein Binding. J.
Chromatogr. A, 988, 1-23 (2003).
2. Porath, J., and Olin, B., Immobilized Metal Ion
Affinity Adsorption and Immobilized
User Guide - RMHE-120K
2 4.1 8.2 12.3 21 82 124 144 206 329
3 12 25 37 62 247 370 432 617 988
4 37 74 111 185 741 1,111 1,296 1,852 2,963
5 111 222 333
Protocol- RMHE-120K
Standard 6 200 µL 100 µL of Standard 7
Standard 5 200 µL 100 µL of Standard 6
Standard 4 200 µL 100 µL of Standard 5
Standard 3 200 µL 100 µL of
S&E Campaign Lab Tools - Tables for laboratory use
–3, 3, 5
[Kr] 4d
105s25p
3
52
127.60
449.51
988
2.01
Te –2, 4, 6
[Kr] 4d
105s25p
4
53
126.90
113.7
184.4
2.21
I –1, 1
Product Information Sheet - CASPASSYRO
Annexin V FLUOS Staining Kit250 tests
03 703 126 001
11 828 690 001
11 828 681 001
11 858 777 001
11 988 549 001
Enzymatic activity PARP cleavage Anti-Poly(ADP-Ribose)Polymerase 11 835 238 001
Caspase
Akt
, B. T., et al. �005. Nat. Rev. Drug Dis. 4, 988.
Samuels, Y., and Ericson, K. �006. Curr. Opin. Oncol. 18, 77.
Bader, A.G., et al. �005. Nat. Rev. Cancer 5, 9�1.
Scheid, M.P., and Woodgett, J.R. �003.
User Guide (IFU) - 1.11151
Factor
m hPa (mbar) m hPa (mbar)
0 1013 1.00 1300 862 1.17
100 1000 1.01 1400 852 1.19
200 988 1.03 1500 841 1.20
300 976 1.04 1600 830 1.22
Apoptosis - Antibodies, Reagents and Kits
siRNA reagent H RNAi 5 nmol M-003002 $375
siRNA plasmid, pKD-Akt3/PKBg-v2 H RNAi 5 µg 62-138 $349
PRAS40 (Proline-Rich Akt Substrate)
Anti-PRAS40 H WB IP M IgG1κ 200 µL 05-988 $309
Anti-phospho-PRAS40
User Guide
Factor
m hPa (mbar) m hPa (mbar)
0 1013 1.00 1300 862 1.17
100 1000 1.01 1400 852 1.19
200 988 1.03 1500 841 1.20
300 976 1.04 1600 830 1.22
Cytoskeletal Signaling Antibodies, Reagents and Kits
PRAS (Proline-Rich AKT Substrate)
Monoclonal Antibody
anti-PRAS40 H IP WB APur M IgG1κ 200 µL 05-988
Polyclonal Antibody
anti-phospho-PRAS40 (Thr246) H M WB APur Rb IgG 100 µL 07-888
Prenylation
ApopTag® Fluorescein Direct In Situ Apoptosis Detection Kit
on the detection of apoptosis in human brain by in situ
end-labeling of DNA. Histochem. J. 27:983-988.
10. Darzynkiewicz, Z., X. Li and J. Gong. (1994) Assays of Cell Viability.
Discrimination of
User Guide
Faktor
m hPa (mbar) m hPa (mbar)
0 1013 1,00 1300 862 1,17
100 1000 1,01 1400 852 1,19
200 988 1,03 1500 841 1,20
300 976 1,04 1600 830 1,22
Calbiochem Biologics 33.2
Opin. Oncol. 18, 77 .
Bader, A .G ., et al . 2005 . Nat. Rev. Cancer 5, 921 .
Hennessy, B .T ., et al . 2005 . Nat. Drug Disc. 4, 988 .
Fruman, D .A . 2004 . Biochem. Soc. Trans. 32, 315 .
Scheid, M .P
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