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Enzyme activity assays

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Lentiviral Titer via p24 (viral coat protein) ELISA Assay
Calculate your lentiviral titer with the p24 ELISA assay. Detailed protcol below.
Enzymatic Assay of Catalase (EC 1.11.1.6)
This procedure may be used for all Catalase products.
Assay Procedure for Xanthine Oxidase Microbial
The appearance of uric acid is measured at 293nm by spectrophotometry.
LPL Activity Assay Protocol
Lipoprotein lipase (LPL) hydrolyzes triglycerides associated with VLDL.
Enzymatic Assay of Esterase
Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
Enzymatic Assay of Aprotinin
Objective: To standardize a procedure for the enzymatic assay of Aprotinin.
Assay Procedure for Protease
This procedure is for informational purposes including assay Procedure, definition, and calculations for Protease
GST Detection Module with CDNB Enzymatic Assay
This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.
Pull-down assays
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)
To standardize a procedure for the enzymatic assay of Diamine Oxidase.
IFN-γ ELISpot Assays on MultiScreen® IP
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
Enzymatic Assay of Ficin (EC 3.4.22.3)
This procedure may be used for all Ficin products.
Enzymatic Assay of Fumarase
Enzymatic Assay of Fumarase
Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)
This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.
Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
Enzymatic Assay of Invertase
Enzymatic Assay of Invertase
Enzymatic Assay of Elastase (EC 3.4.21.36)
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
Enzymatic Assay of Pepsin (3.4.23.1)
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
Enzymatic Assay of Mutanolysin
This procedure may be used for Mutanolysin products.
Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)
To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.
Enzymatic Assay of Peroxidase (EC 1.11.1.7)
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
MTT Assay Protocol for Cell Viability and Proliferation
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
Enzymatic Assay of β-AMYLASE (EC 3.2.1.2)
Beta-amylase is found in bacteria, fungi, and plants. To measure β-amylase activity, this assay uses a colorimetric spectrophotometric stop reaction at 540 nm.
Enzymatic Assay of Chloramphenicol Acetyltransferase
To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.
Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)
Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)
Enzymatic Assay of Lysozyme (EC 3.2.1.17)
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
Enzymatic Assay of 5’-Nucleotidase
To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.
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