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Applications:Low pressure liquid chromatography

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Description of viral clearance using Capto adhere from Cytiva.

Maintenance of Multimodal Chromatography Media and Storage Conditions

This page describes the maintenance of media and storage conditions for multimodal chromatography using Cytiva products.

Optimization of Loading Conditions on Capto Adhere Using DoE

This page describes the optimization of loading conditions on Capto adhere from using full factorial design of experiments (DoE).

Purification of Thiol-Containing Substances By Covalent Chromatography

Thiol-containing substances can be isolated selectively by covalent binding to an activated thiolated matrix via thiol-disulphide exchange to form a mixed disulphide bond. After washing away unbound material, the thiol-containing substance is eluted by reducing the disulphide bond. This technique

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Characteristics of Dextrin Sepharose® High Performance Products

This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protein G and Protein A Bind to Different IgG

This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.

Ion Exchange Chromatography

Ion exchange chromatography is employed by the Genopure Plasmid Midi Kit and the Genopure Plasmid Maxi Kit.

UHPLC Analysis of Ephedrine and Related Compounds on Supel™ Carbon LC Column

Ultra-high performance liquid chromatographic (UHPLC) separation of ephedrine and its three structural analogs based on polar retention effect on graphite (PREG) mechanism using Supel™ Carbon LC column.

UHPLC Analysis of Paraquat and Diquat on Supel™ Carbon LC

Ultra-high performance liquid chromatographic (UHPLC) separation of polar herbicides, paraquat and diquat using SupelTM Carbon LC column with baseline resolution, excellent peak shape, and good lot-to-lot reproducibility.

UHPLC Analysis of Nucleosides on Supel™ Carbon LC Column: Performance Comparisons with Competitor

Ultra-high performance liquid chromatographic (UHPLC) separation of 12 nucleosides using Supel™ Carbon LC column in 15 minutes for use in identification and quantitation of nucleoside biomarkers.

LC-MS/MS Analysis of Bile Acids using Ascentis® Express C18

Isobaric separation of bile acids and conjugates by LC-MS/MS on Ascentis® Express C18 column with excellent resolution and linearity.

Determination of Chlorogenic acid and Baicalin in Compounded Lonucerae Japonicae Flos using a Discovery® HS C18 Column

Extraction and analyses of chlorogenic acid and baicalin active ingredients in traditional Chinese medicine (TCM) according to Chinese pharmacopeia monograph by HPLC with Discovery® C18 column to check: chromatographic data, linearity, specificity and repeatability, LOQ and LOD

HPLC analysis of Letrozole and its impurities by following the USP Guidelines Using a Purospher® STAR RP18e Column and UV Detection

An application note concerning the HPLC analysis of letrozole and its impurities by following the USP43-NF38 Guidelines using a Purospher® STAR RP18e Column.

Determination of Ephedrine HCl and Pseudoephedrine HCl in Xiao’er Kechuanling Oral Solution with Purospher® STAR RP-18e colμmn

HPLC method to analyze ephedrine HCl and pseudoephedrine HCl in traditional Chinese medicine (TCM) with a Purospher® RP-18e colμmn to report chromatographic data, linearity, specificity and repeatability and LOD and LOQ.

HPLC Analysis of Fenofibrate and Its Impurities by Following the USP43-NF38 Guidelines Using a Purospher® STAR RP18e Column

An application note concerning the HPLC analysis of fenofibrate and its impurities by following the USP43-NF38 Guidelines using a Purospher® STAR RP18e Column.

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Polishing of MAbs Using Capto Adhere ImpRes in Bind/Elute Mode

In these studies, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute mode was evaluated.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Purification using GST SpinTrap™

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

UHPLC Analysis of Vitamin D2 & D3 Metabolites and Epimers on Supel™ Carbon LC Columns

Ultra high-performance liquid chromatographic (UHPLC) separation of Vitamin D Metabolites― 3-epi-25-hydroxyvitamin D2 and 25-hydroxyvitamin D2, using Supel™ Carbon LC column with baseline separation, excellent peak shape, and sensitivity.

LRA (Lipid Removal Agent) for Biopharmaceutical Production

Lipids are naturally-occurring hydrocarbons that are poorly soluble in water and soluble in non-polar solvents such as ether and chloroform.

QuEChERS Cleanup and Analysis of Veterinary Drugs in Milk by LC/MS/MS

Protein Affinity Chromatography

Affinity chromatography is the process of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. This process allows for the highly specific and efficient purification of many diverse proteins and other compounds. The process requires

Lewatit® Ion Exchange Resins – All-around Solutions for Chemical Processes

Chemical processes comprise the steps of processing chemical starting materials, synthesizing the products, isolating the generated materials from the reaction mixture and subsequent purification.

Comparison of Spherical and Irregular Silicas in Flash Chromatography

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature

Troubleshooting Reversed Phase Chromatography (RPC)

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

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