Nucleic acid labeling and detection

Applied Filters:

facet applications:Nucleic acid labeling and detection

facet content type:Protocol

Showing 1-12 of 12 results

DIG RNA Labeling Mix Protocol Troubleshooting

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).

NBT/BCIP Stock Solution Protocol & Troubleshooting

NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting

Dextran Sulfate

Dextran sulfates as sodium salts are water-soluble and stable, containing approximately 17% sulfur for various applications.

DIG Easy Hyb™ Granules Protocol & Troubleshooting

NBT Protocol

DIG RNA Labeling Kit (SP6/T7) Protocol & Troubleshooting

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).

NBT Protocol & Troubleshooting

There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

DIG Northern Starter Kit Protocol & Troubleshooting

DNA probes, like PCR or random-primed DNA probes, are effective for northern blots, particularly for detecting abundant messages.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Oligonucleotide Melting Temperature

Learn about methods for calculating oligonucleotide melting temperature (Tm).