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Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
Optimized PCR-based Detection of Mycoplasma
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
Buccal DNA Extraction & WGA Amplification Protocol
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
Expand™ Long Template PCR System Protocol Troubleshooting
This polymerase mixture produces a high yield of PCR product from genomic DNA.
RNA Handling When Using the First Strand cDNA Synthesis Kit for RT-PCR (AMV)
First Strand cDNA Synthesis Kit for RT-PCR (AMV).
GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol
GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA
Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
TeloTAGGG Telomerase PCR ELISA Protocol & Troubleshooting
The TeloTAGGG Telomerase PCR ELISA is used for sensitive qualitative detection of telomerase activity.
Multiplex Real-Time PCR
Multiplex qPCR employing probe-based chemistries is a demanding application that often requires extensive optimization and validation.
Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
HighPrep™ PCR Clean Up Kit and Purification System Protocol
HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.
Reverse Transcription Protocol Using SYBR Green Dye Detection
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)
MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.
End Point PCR Protocol for Long and Accurate DNA Amplification
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
SEQR - SeqPlex RNA Amplification Kit Protocol
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
Amplification of Genomic DNA using REDTaq® DNA Polymerase
Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.
Extract-N-Amp™ Tissue PCR Kit Protocol
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Annealing Oligonucleotides Protocol
Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences.
Extract-N-Amp™ Plant PCR Kits Protocol
The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.
Plant-Based PCR Inhibitors & Master Mix Performance
Evaluate PCR inhibitors and enzyme selection using GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix, and competitors.
Long & Accurate PCR with RedAccuTaq®
REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.
WGA Using GenomiPhi™
GenomiPhi™ method from Cytiva offers isothermal DNA amplification without a thermal cycler, using Phi29 DNA polymerase.
DNA Extraction & WGA Amplification from Soil Samples
GenomePlex® amplification improves DNA yield from soil samples, ensuring downstream analysis reliability despite potential nuclease damage.
Saliva DNA Extraction & WGA Amplification
Whole Genome Amplification products, including kits for DNA extraction, support various DNA sources.
Hot Start dNTP Protocol
Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.
Extraction & Amplification of Whole Blood Using WGA-Protocol
Simple DNA isolation protocol from whole blood samples for genomic analysis, followed by GenomePlex® amplification.
Animal Tissue DNA-Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.
Whole Genome Amplification: Blood Card Protocol
Efficient extraction of genomic DNA from blood cards for whole genome amplification and analysis.
Standard Reverse Transcription Protocol
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
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