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A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®
Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.
Restorase® DNA Polymerase
Restorase® was developed for researchers unable to achieve amplification of damaged DNA templates when using other commercially available DNA polymerases.
PCR Assay Optimization and Validation
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
Colony PCR
Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.
Whole Transcriptome Amplification of RNA from Low Cell-Number Samples
The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.
ThermaStop™-RT PCR Additive
PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.
Optimizing Crude Plant Samples for PCR
Overcoming potent inhibitors and sample diversity in direct plant PCR.
5'/3' RACE Kit, 2nd Generation Troubleshooting
In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction
Long and Accurate PCR
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
Polymerase Chain Reaction (PCR) Basics
Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.
Complete Solutions for PCR Assay Development
Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.
Extract-N-Amp™ Product Guide
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Oligonucleotide Quality Control & Quality Assurance
The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification.
Dual-Labeled Probes in Digital PCR
Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.
Custom LAMP Primers in Partnership with New England Biolabs
Primers manufactured using proprietary purification adjustments to perform optimally in loop-mediated isothermal amplification assays.
KAPA Long Range PCR Kits FAQs
Review some of the most asked questions regarding the KAPA Long Range PCR system, which is a blend of Taq DNA polymerase and an engineered archaeal DNA polymerase with proofreading capability.
KAPA3G Plant PCR Kits FAQs
Amplify up to 5 kb fragments from purified plant DNA extracted using commercial kits or CTAB-based methods. Review some of the most asked questions regarding direct PCR from leaf disks, seeds and other plant tissues.
Mycoplasma PCR ELISA Protocol
Mycoplasmas are potential contaminants in mammalian cell culture manufacturing. All products produced in cell culture to be tested for the presence of Mycoplasma
Extract-N-Amp™ Blood PCR Kit Protocol
The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.
Extract-N-Amp Reagent: Effective PCR from FTA® Blood Cards
Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.
RT-PCR Troubleshooting
PCR troubleshooting guide for reliable data in molecular assays.
KAPA2G Fast PCR Kits FAQ
Fast PCR reduces reaction time, enhancing efficiency with optimized cycling parameters.
PCR Technologies Guide
Basic PCR/qPCR/dPCR protocols for assay quality control and specific studies.
Water for Nuclease-sensitive Molecular Biology Studies
Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.
Cloning Troubleshooting
Molecular cloning inserts genes into plasmid vectors for protein expression in different studies.
Extract-N-Amp™ Tissue Feature
Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.
MIQE: Real-Time PCR Experiment Standards
MIQE-compliant qPCR services support primer design, protocol development, and data analysis for faster product development and publication.
Array CGH Analysis with Whole Genome Amplification
aCGH technology evolves for high-res chromosomal analysis, needing a large DNA input per sample, advancing genetic research.
WTA2 Amplicon Modification for NGS
Transplex Whole Transcriptome Amplification (WTA2) precisely amplifies RNA maintaining transcript levels in test and reference samples.
Nuclease-Free Water at Your Fingertips
An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.
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