HomeSmall Molecule HPLCAscentis® Express F5 (Pentafluorophenyl) U/HPLC Columns

Ascentis® Express F5 (Pentafluorophenyl) U/HPLC Columns

Enhanced selectivity for stereoisomers and closely related compounds

Our Ascentis® Express F5 column brings together the highly efficient, robust Fused-Core® particle technology with F5 chemistry for the successful separation of polar and non-polar organic compounds.

The pentafluorophenylpropyl (PFP) stationary phase of the Ascentis® Express F5 column, based on Fused-Core® particle technology, provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming p-p and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis® Express F5 columns can be used for basic, acidic, or neutral compounds with alternate selectivity from C18 phases.

Learn More About Fluorinated Stationary Phases:

  • Alternative Retention and Selectivity Using Fluorinated Stationary Phases
Hydrocortisone and Prednisolone
Comparison of C18 and F5

Comparision of C18 and F5 for the Separation of Closely Related Steroids

Featured Applications:

Ascentis® Express F5 is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core® particle design. The Fused-Core® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths. 3 different particle sizes are available for your specific needs:

2.0 µm particles

Best Fused-Core UHPLC Column

An optimized solution for high-throughput small molecule analysis

2.7 µm particles

Fast HPLC on ANY System

A practical solution that delivers UHPLC performance from any HPLC

5.0 µm particles

The Lab Work-Horse Column

True plug and play solution for improving existing 3 or 5 µm fully porous particle HPLC columns

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