Merck
All Photos(7)

07-329

Sigma-Aldrich

Anti-acetyl-Histone H4 (Lys16) Antibody

Upstate®, from rabbit

Synonym(s):
Histone H4 Acetylated on Lysine 16, H4K16Ac, Histone H4 (acetyl K16), H4 histone family, member A, H4a
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, mouse

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
dot blot: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... H4C1(8359)
mouse ... H4C1(326619)

General description

Histone H4, Acetylated on Lysine 16 (UniProt: P62805; also known as H4K16Ac, Histone H4 (acetyl K16), H4 histone family, member A, H4a) is encoded by the HIST1H4A (also known as H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, HIST1H4I, H4/N, HIST1H4B) gene (Gene ID: 121504, 554313, 8294, 8359, 8360, 8361, 8362, 8363, 8364, 8365, 8366, 8367, 8368, 8370) in human. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 features a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone H4 are known to be acetylated on Lysine 5, 8, 12, and 16. Acetylations are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Hyper-acetylation of the histone tails is shown to neutralize positive charge and weakens histone-DNA and nucleosome-nucleosome interactions that destabilizes chromatin structure and increases the accessibility of DNA to various DNA-binding proteins. Chromosomal aberrations involving histone H4 is a cause of B-cell non-Hodgkin lymphomas.

Specificity

Broad species cross-reactivity is expected.
This rabbit polyclonal antibody detects human Histone H4 acetylated on Lysine 16.

Immunogen

KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H4 acetylated on Lysine 16.
Epitope: N-terminus (Lys 16)

Application

Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Chromatin Immunoprecipitation Analysis: 1 ug from a representative lot immunoprecipitated Acetyl-Histone H4 (Lys16) in HeLa chromatin.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in HeLa, A431, HUVEC, and NIH/3T3 cell lines.

Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in

an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Anti-acetyl-Histone H4 (Lys16) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H4 (Lys16) also known as H4K16Ac, Histone H4 (acetyl K16 & has been published and validated in ChIP, WB, Mplex, PIA, DB, ChIP-seq.

Quality

Evaluated by Western Blotting in lysate from Sodium Butyrate treated HeLa cells.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Acetyl-Histone H4 (Lys16) in lysates from Sodium Butyrate treated HeLa cells.

Target description

10 kDa

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8° C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Genomewide screen for negative regulators of sirtuin activity in Saccharomyces cerevisiae reveals 40 loci and links to metabolism.
Raisner, RM; Madhani, HD
Genetics null
Characterization of BEAF mutations isolated by homologous recombination in Drosophila.
Roy, S; Gilbert, MK; Hart, CM
Genetics null
Long-range spreading of dosage compensation in Drosophila captures transcribed autosomal genes inserted on X.
Gorchakov, AA; Alekseyenko, AA; Kharchenko, P; Park, PJ; Kuroda, MI
Genes & Development null
Adjacent gene pairing plays a role in the coordinated expression of ribosome biogenesis genes MPP10 and YJR003C in Saccharomyces cerevisiae.
Arnone, JT; McAlear, MA
Eukaryotic Cell null
Marnie E Gelbart et al.
Nature structural & molecular biology, 16(8), 825-832 (2009-08-04)
The Drosophila melanogaster male-specific lethal (MSL) complex binds the single male X chromosome to upregulate gene expression to equal that from the two female X chromosomes. However, it has been puzzling that approximately 25% of transcribed genes on the X

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