Absorbance (1.0 M, H2O, 260 nm):≤0.05.
Absorbance (1.0 M, H2O, 280 nm): ≤0.04
A zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pKa of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.
A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.
25 g in Plastic ampoule
5 kg in Fibre carton
100, 500 g in Glass bottle
1 kg in Glass bottle
Toxicity: Irritant (B)
Following reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany