391340

HEPES, Free Acid, Molecular Biology Grade - CAS 7365-45-9 - Calbiochem

A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.

• Synonym(s): N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, HEPES
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• MDL number: MFCD00006158

Properties

• Quality Level: 100
• assay: ≥99% (alkalimetric)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubility: water: 400 mg/mL; aqueous base: soluble
• cation traces: heavy metals: ≤0.0005%
• foreign activity: DNase, RNase, and protease, none detected
• shipped in: ambient
• storage temp.: 15-25°C
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pK_a of 7.48 at 25°C.

Packaging

25 g in Glass bottle

250 g in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable