IQ motif and SEC7 domain-containing protein 3 (UniProt: Q76M68; also known as Potential synaptic guanine nucleotide exchange factor for Arf, SynArfGEF-Po) is encoded by the Iqsec3 (also known as Sag) gene (Gene ID: 404781) in rat. IQSEC3 along with IQSEC1 and IQSEC2 constitutes a family of brefeldin A-resistant ARF guanine nucleotide exchange factors (GEFs). It is a cytoplasmic protein that is expressed in the brain and is localized to dendrites and soma. Its expression is reported as early as E18 in hippocampal neurons. It is a key factor in controlling network activity in the hippocampus under both physiological and pathological conditions. It is shown to be exclusively localized to inhibitory synapses and it directly binds to gephyrin to promote inhibitory synapse formation in an ARF-GEF activity-dependent manner. IQSEC3 is reported to regulate GABAergic synapse maintenance in vivo and it links gephyrin-GABAA receptor complexes with ARF-dependent pathways to prevent aberrant, runaway excitation. Knockdown of Iqsec3 gene in the hippocampal dentate gyrus (DG) induces a significant reduction in GABAergic synapse density and causes a massive reduction of somatostatin expression in the DG hilus. IQ domain of IQSEC3 is localized to amino acids 311-340; the SEC7 domain is in amino acids 646-838; and the PH domain is in amino acids 852-985. (Ref.: Kim, S., et al. (2020). Cell Rep. 30(6); 1995-2005; Um, JW., et al. (2016). J. Biol. Chem. 291(19); 10119-10130).
This rabbit polyclonal antibody detects IQ motif and SEC7 domain-containing protein 3 (IQSEC3). It targets an epitope within the N-terminal domain.
GST-tagged recombinant fragment corresponding to the first 185 amino acids from rat IQ motif and SEC7 domain-containing protein 3 (IQSEC3).
Quality Control Testing
Evaluated by Western Blotting in Rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IQSEC3 in Rat brain tissue lysates.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IQSEC3 in mouse brain tissue lysate.
Immunohistochemistry Applications: A representative lot detected IQSEC3 in Immunohistochemistry applications (Kim, S., et. al. (2020). Cell Rep. 30(6):1995-2005).
Immunocytochemistry Analysis: A representative lot detected IQSEC3 in Immunochemistry applications (Um, JW., et. al. (2016). J. Biol. Chem. 291(19):10119-30).
Immunoprecipitation Analysis: A representative lot detected IQSEC3 in Immunoprecipitation applications (Um, JW., et. al. (2016). J. Biol. Chem. 291(19):10119-30).
Western Blotting Analysis: A representative lot detected IQSEC3 in lysates from HEK293T cells transfected with Flag-IQSEC3, but not in untransfected cells. (Data Courtesy of Dr Jaewon Ko & Dr Seungjoon Kim at Daegu Gyeongbuk Institute of Science and Technology (DGIST)
Western Blotting Analysis: A representative lot detected IQSEC3 in Western Blotting applications (Kim, S., et. al. (2020). Cell Rep. 30(6):1995-2005).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IQSEC3, Cat. No. ABN2294, is a rabbit polyclonal antibody that detects IQSEC3 and is tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Purified rabbit polyclonal antibody in 10 mM Tris-HCl pH 7.5 with 0.05% sodium azide.
Storage and Stability
Recommended storage: +2°C to +8°C.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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