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IEVH85R
Immobilon®-E PVDF Membrane
The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
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Synonym(s):
Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane
eCl@ss:
32031613
NACRES:
NB.23
Recommended Products
material
PVDF membrane
plain filter
white filter
feature
easily wetted with water
manufacturer/tradename
Immobilon®
technique(s)
western blot: suitable
filter L × W
8.5 cm × 10 m
pore size
0.45 μm pore size
compatibility
for use with Amido black
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Transillumination
detection method
chemiluminescent
colorimetric
radioactive
shipped in
ambient
Related Categories
General description
Filter Code: IEVH
Immobilon-E is the first PVDF membrane for Western blotting that can be wetted with with aqueous transfer buffer. This eliminates the alcohol pre-wet step required with other PVDF membranes.
Primary Binding Mechanisms: Electrostatic, hydrophobic
This 8.5cm x 10m roll offers 1-cut convenience for standard mini-blots (7 x 8.5cm) and midi-blots (8.5 x 13.5cm). It works with the optional Immobilon NOW Dispenser (catalog number IMDISP).
Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
- Compatible with chemiluminescent and chromogenic detection
Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes.
Features & Benefits:
- High protein binding provides stronger signal
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be stripped and reprobed multiple times
Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
- Compatible with chemiluminescent and chromogenic detection
Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes.
Features & Benefits:
- High protein binding provides stronger signal
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be stripped and reprobed multiple times
Packaging
1 ea
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificate of Analysis
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Certificate of Quality
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