MAB2514

Anti-Integrin αVβ1 Antibody

CHEMICON^®, rat monoclonal, BMB5

• Synonym(s): VLA-5
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: BMB5, monoclonal
• Species reactivity: mouse
• Application: FACS, IHC, IP, WB
• Citations: 13

Properties

• Product Name: Anti-Integrin α5β1 Antibody, clone BMB5, ascites fluid, clone BMB5, Chemicon^®
• biological source: rat
• Quality Segment: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: BMB5, monoclonal
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG3
• NCBI accession no.: NM_002205.2
• UniProt accession no.: P08648
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGA5(3678)

Description

Immunogen

The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.

Application

Inhibition of cell adhesion to fibronectin: 10 μg/mL

Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMB5 alone may not be complete.

Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μg

Flow cytometry: 10μg/mL

Western blot (non-denaturing gel)

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

This Anti-Integrin α5β1 Antibody, clone BMB5 is validated for use in FC, IP, WB, IH, FUNC for the detection of Integrin α5β1.

Biochem/physiol Actions

Antibody MAB2514 was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.

Physical form

Liquid, containing no preservatives.

Preparation Note

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable