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MABC1634

Sigma-Aldrich

Anti-MLKL Antibody, clone 5A6

new
Synonym(s):
Mixed lineage kinase domain-like protein

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

5A6, monoclonal

mol wt

calculated mol wt 54.32 kDa
observed mol wt ~54 kDa

purified by

using protein G

species reactivity

mouse

packaging

antibody small pack of 100 μL

technique(s)

immunofluorescence: suitable
western blot: suitable

isotype

IgG2aλ

epitope sequence

C-terminal

Protein ID accession no.

UniProt accession no.

shipped in

ambient

Gene Information

mouse ... Mlkl(74568)

Related Categories

General description

Mixed lineage kinase domain-like protein (UniProt: Q9D2Y4; also known as MLKL) is encoded by the Mlkl gene (Gene ID: 74568) in murine species. MLKL is a pseudokinase that is a terminal protein in the pro-inflammatory necroptotic cell death program. It plays a critical role in TNFa-induced necroptosis. It is highly expressed in thymus, colon, intestine, liver, spleen, and lung. Its expression is much lower in skeletal muscle, heart and kidney, and it is not detected in the brain. Although it contains a protein kinase domain (aa 192-456), it lacks several residues that are essential for protein kinase activity. It is activated following phosphorylation by RIPK3, which leads to its homotrimerization and localization to the plasma membrane where it binds to highly phosphorylated inositol (InsP6) and disrupts membrane integrity to cause necrotic cell death. Phosphorylation of MLKL at serine 345 is shown to be essential for necroptosis in murine cells. Its interaction with RIPK3 is species specific and mouse MLKL does not interact with human RIPK3. In contrast to human protein, mouse MLKL is not inhibited by necrosulfonamide, because at position 85 it contains a tryptophan residue instead of a cysteine. Under basal conditions, MLKL is shown to reside in small puncta that are distributed evenly throughout the cytoplasm, but it is detected in plasma membrane shortly after its phosphorylation. It contains two coiled coil regions (aa 61-81 and 138-229) and the second coiled coil region is responsible for its oligomerization. Two isoforms of MLKL have been described that are produced by alternative splicing. (Ref.: Samson, AL., et al. (2021). Cell Death Differ. 28(7); 2126-2144; Wang, H., et al (2014). Mol. Cell 54(1); 133-146).

Specificity

Clone 5A6 is a rat monoclonal antibody that detects murine Mixed lineage kinase domain-like protein (MLKL). It targets an epitope within the C-terminal region.

Immunogen

His-tagged full-length recombinant mouse Mixed lineage kinase domain-like protein (MLKL).

Application

Quality Control Testing

Evaluated by Western Blotting in lysates from Mouse dermal fibroblasts.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected MLKL in lysates from wild-type Mouse dermal fibroblasts, but not in lysates from fibroblasts with Mlkl knockout.

Tested Applications

Immunofluorescence Analysis: A representative lot detected MLKL in Immunofluorescence applications (Samson, A.L., et al. (2021). Cell Death Differ. 28(7):2126-2144).

Western Blotting Analysis: A representative lot detected MLKL in Western Blotting applications (Samson, A.L., et al. (2021). Cell Death Differ. 28(7):2126-2144).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-MLKL, clone 5A6, Cat. No. MABC1634, is a rat monoclonal antibody that detects MLKL and is tested for use in Immunofluorescence and Western Blotting.

Physical form

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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