Merck
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KK4752

Roche

KAPA PROBE FAST One-Step

Universal

Synonym(s):
RT-qPCR, qPCR, probe qPCR
NACRES:
NA.55

Quality Level

usage

sufficient for 500 reactions

feature

dNTPs included: no
hotstart: no

packaging

pkg of 500 RXNS

manufacturer/tradename

Roche

technique(s)

RT-qPCR: suitable

input

purified DNA

detection method

probe-based

storage temp.

−20°C

General description

KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal Kit is a sensitive and convenient solution for real-time PCR using RNA as template. The kit is designed for high-throughput, fast-cycling, one-step RNA quantification using sequence-specific fluorogenic probes. It is compatible with all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g. TaqMan®) and displacement probes (e.g., molecular beacons)

Application

KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal has been used for:
  • Gene expression analysis
  • SNP/mutation analysis
  • Gene knockdown validation
  • RT-PCR

Features and Benefits

Quick Notes:
  • The kit is suitable for all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan) and displacement probes (e.g., molecular beacons).
  • Use only gene-specific primers for one-step qRT-PCR.
  • Optimal cDNA synthesis is achieved at 42°C for 5 min.
  • 3 min at 95°C is sufficient for RT inactivation and DNA polymerase activation.
  • Do not exceed 25 μL reaction volumes

Packaging

Kit includes qPCR Master Mix (2X), KAPA RT Mix (50X), dUTP (10 mM), ROX High Reference Dye (50X) and ROX Low Reference Dye (50X)

Quality

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination.

Preparation Note

Always ensure that components have been fully thawed and thoroughly mixed before use. The KAPA PROBE FAST qPCR Master Mix may not freeze solidly, even when stored at -20 °C. The KAPA RT Mix is temperature-sensitive, and should be stored at -20 °C and kept on ice during use. ROX reference dye is light-sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Simultaneous diagnosis of Cetacean morbillivirus infection in dolphins stranded in the Spanish Mediterranean sea in 2011 using a novel Universal Probe Library (UPL) RT-PCR assay.
Rubio-Guerri C, et al.
Veterinary Microbiology, 165(1-2), 109-114 (2013)

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