Merck
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51558

Sigma-Aldrich

HEPES buffered saline

BioUltra, for molecular biology, 2× concentrate

MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

grade

for molecular biology

product line

BioUltra

form

liquid

quality

2× concentrate

composition

dextrose, 2.0 g/L
HEPES, 10 g/L
KCl, 0.74 g/L
NaCl, 16 g/L
Na2HPO4.2H2O, 0.27 g/L

concentration

2 ×

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.339

pH

7.2 (25 °C)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Application

HEPES buffered saline has been used:
  • for the preparation of plasmid DNA
  • as a reagent to prepare transfection master mix for DNA construct vectors
  • to develop hydrogel precursor for cell leakage assay

Components

concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

Quotes and Ordering

A new approach to neural cell culture for long-term studies
Potter SM and DeMarse TB
Journal of Neuroscience Methods, 110(1-2), 17-24 (2001)
Calcium phosphate transfection.
Kingston RE, Chen CA, Okayama H.
Current Protocols in Immunology, 10, 10-10 (2001)
Labeling embryonic mouse central nervous system cells by in utero electroporation
Tabata H and Nakajima K
Development, Growth & Differentiation, 50(6), 507-511 (2008)
C P Leamon et al.
Bioconjugate chemistry, 10(6), 947-957 (1999-11-24)
Poly(ethylene glycol) of various sizes was used as a molecular spacer to separate the cell-targeting ligand, folate, from the surface of poly-L-lysine. The resulting ternary macromolecule (pLys-PEG-folate) was investigated in various formulations for its ability to transfect reporter plasmids into
J. Sambrook et al.
Molecular Cloning: A Laboratory Manual, B11 (1989)

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