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69385

Supelco

Protein Standard Mix 15 - 600 kDa

for size exclusion chromatography

NACRES:
NA.24

Quality Level

form

neat

analyte chemical class(es)

amino acids, peptides, proteins

application(s)

gel permeation chromatography (GPC): suitable

Featured Industry

Food and Beverages

format

neat

storage temp.

−20°C

General description

contains Ribonuclease A, Thyroglobulin, γ-Globulin, Albumin and p-aminobenzoic acid (pABA)
The protein standard mix is a calibration standard to test and monitor performance of size exclusion chromatography (SEC) columns. It is a lyophilized mixture of molecular weight markers ranging from 15 kDa to 600 kDa.

Application

Protein Standard Mix 15-600 kDa has been used as a molecular weight calibration standard for the determination of nitrated and oligomerized proteins by size exclusion high-performance liquid chromatography with photodiode array (SEC-HPLC-PDA) detector.

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

Beata Maria Walter et al.
Protein expression and purification, 169, 105587-105587 (2020-02-01)
Prs (phosphoribosyl pyrophosphate synthase) is a broadly conserved protein that synthesises 5-phosphoribosyl 1-pyrophospate (PRPP); a substrate for biosynthesis of at least 10 enzymatic pathways including biosynthesis of DNA building blocks - purines and pyrimidines. In Escherichia coli, it is a...
Robert C Monsen et al.
Nucleic acids research, 48(10), 5720-5734 (2020-02-23)
The structure of the 68 nt sequence with G-quadruplex forming potential within the hTERT promoter is disputed. One model features a structure with three stacked parallel G-quadruplex units, while another features an unusual duplex hairpin structure adjoined to two stacked...
Simultaneous determination of nitrate and oligomerized proteins by size exclusion high-performance liquid chromatography coupled to photodiode array detection.
Liu F, et al.
Journal of Chromatography A, 1495, 76-82 (2017)
Chunjun Yan et al.
Food research international (Ottawa, Ont.), 141, 110163-110163 (2021-03-02)
This study investigated the effects of walnut phenolics and extraction methods on the composition and structural properties of walnut protein isolates (WPIs). Fluorescence quenching experiments showed that walnut phenolics could bind to walnut globulins, albumins, and glutelins with apparent affinity...
Andrew J Bowling et al.
Toxins, 11(5) (2019-05-19)
Vip3A proteins are important for the control of spodopteran pests in crops, including Spodoptera frugiperda (fall armyworm). Native Vip3Ab1 controls S. frugiperda, but it is ineffective against S. eridania (southern armyworm), a major pest of soybean in South America. Recently...

Articles

Columns and Analytical Standards for Protein SEC

Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.

Protocols

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Calibration Curves for TSKgel® SW mAb Columns

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