Merck
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H23830

Sigma-Aldrich

HEPES

reagent grade, ≥99% (titration), crystalline

Synonym(s):
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, HEPES
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
MDL number:

Quality Level

biological source

synthetic

grade

reagent grade

assay

≥99% (titration)

form

crystalline

useful pH range

6.8-8.2

pKa (25 °C)

7.5

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[7] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[1]

Application

HEPES has been used:
  • As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo†
  • As a component of Hanks′ solution used in the degradation testing of Iron†
  • As a component of HEPES medium in the transfection of cell culture with plasmids†
  • Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells†
  • As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies†

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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