Merck
All Photos(1)

Documents

43816

Sigma-Aldrich

DL-Dithiothreitol solution

BioUltra, for molecular biology, ~1 M in H2O

Sign Into View Organizational & Contract Pricing

Synonym(s):
Cleland′s reagent, (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, (±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
Empirical Formula (Hill Notation):
C4H10O2S2
CAS Number:
Molecular Weight:
154.25
Beilstein:
1719757
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

Assay

900-1100 mmol/L (Titration molarity)

form

liquid

reaction suitability

reagent type: reductant

concentration

~1 M in H2O

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH

2.7-5.0

density

1.04 g/mL at 20 °C

absorption

cut-off at 330 nm

suitability

in accordance for filter test
suitable for molecular biology

storage temp.

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
43819D9163646563
DL-Dithiothreitol solution BioUltra, for molecular biology, ~1 M in H2O

Sigma-Aldrich

43816

DL-Dithiothreitol solution

Premium Grade
DL-Dithiothreitol ≥99.0% (RT)

Sigma-Aldrich

43819

DL-Dithiothreitol

-
DL-Dithiothreitol suitable for electrophoresis, ≥99%

Sigma-Aldrich

D9163

DL-Dithiothreitol

-
DL-Dithiothreitol solution 1 M in H2O

Supelco

646563

DL-Dithiothreitol solution

-
product line

BioUltra

product line

-

product line

-

product line

-

assay

900-1100 mmol/L (Titration molarity)

assay

≥99.0% (RT)

assay

≥99%

assay

-

form

liquid

form

powder

form

powder

form

liquid

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

≤0.3% 4,5-dihydroxy-1,2-dithiane

impurities

-

impurities

-

pH

2.7-5.0

pH

-

pH

-

pH

-

General description

Dithiothreitol (DTT), a thiol group protectant, is an excellent reagent for preventing the oxidation of SH groups in proteins. DTT solution is a potent and versatile disulfide-reducing agent. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange.

Application

DL-dithiothreitol solution has been used:

  • in the treatment of intestinal explants
  • in homogenate preparation of gill cells for enzymatic assays
  • in protein electrophoresis for western blotting
  • in transglutaminase activity
  • in sample buffer for SDS-PAGE
  • in Western Blot, as a lysis buffer component for single-nucleus RNA-sequencing
  • as a component of protein extraction buffer for cell lysis

Biochem/physiol Actions

DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms. It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.Dithiothreitol (DTT) is broadly utilized in synthetic peptide synthesis and biochemical arrangements of thiol proteins. It can also be used to study protein chemistry topics like enzyme activity and protein folding. DTT explicitly controls the thiol-disulfide exchange response to completely reduce the intra-or between sub-atomic disulfide bonds in biomolecules. Thiols and the cyclic disulfide of DTT are the products of this reaction.

Features and Benefits

  • BioUltra grade - 1M DTT solution, suitable for molecular biology
  • Free from DNases, RNases, phosphatases, and proteases

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 5

1 of 5

L-(−)-Dithiothreitol ≥95% (titration)

Sigma-Aldrich

D9760

L-(−)-Dithiothreitol

Protector RNase Inhibitor

Roche

RNAINH-RO

Protector RNase Inhibitor

Spermidine BioUltra, for molecular biology, ≥99.5% (GC)

Sigma-Aldrich

85558

Spermidine

Expression and Localization of Cathepsins B, D, and G in Dupuytren?s Disease
Tan K, et al.
Plastic and reconstructive surgery. Global open, 6(2), e0133549-e0133549 (2018)
New Technologies, An Issue of Orthopedic Clinics (2018)
Suppression of GABA B receptor function in vivo by disulfide reducing agent, dl-dithiothreitol (DTT)
Carai M A M, et al.
Psychopharmacology, 174(2), 283-290 (2004)
In Utero Exposure to Maternal Diabetes Is Associated With Early Abnormal Vascular Structure in Offspring
Dib A, et al.
Frontiers in physiology, 9, 350-350 (2018)
Delivery of human immunodeficiency virus vaccine vectors to the intestine induces enhanced mucosal cellular immunity
Wang L, et al.
Journal of Virology, 83(14), 7166-7175 (2009)

Articles

Bis-Tris gels and buffers for superior protein resolution compared to traditional tris-glycine gels.

Protocols

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service