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57180

Sigma-Aldrich

Indo 1

for fluorescence, ≥80.0% (HPLC)

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Synonym(s):
4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid pentapotassium salt
Empirical Formula (Hill Notation):
C32H26K5N3O12
CAS Number:
132319-56-3
Molecular Weight:
840.05
MDL number:
MFCD00077797
PubChem Substance ID:
329758276
NACRES:
NA.32

grade

for fluorescence

Assay

≥80.0% (HPLC)

form

solid

fluorescence

λex 331 nm; λem 398 nm in 0.1 M Tris pH 8.0, 10 mM Ca2+
λex 349 nm; λem 482 nm (100mM KCI)

suitability

in accordance for fluorescence

storage temp.

−20°C

SMILES string

[K+].[K+].[K+].[K+].[K+].Cc1ccc(N(CC([O-])=O)CC([O-])=O)c(OCCOc2cc(ccc2N(CC([O-])=O)CC([O-])=O)-c3cc4ccc(cc4[nH]3)C([O-])=O)c1

InChI

1S/C32H31N3O12.5K/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22;;;;;/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45);;;;;/q;5*+1/p-5

InChI key

VGZPRMSXVNDMAN-UHFFFAOYSA-I

Application

Indo 1, a fluorescent calcium chelator and indicator, is used to monitor cytoplasmic Ca2+ transients induced by various physiological and mechanical processes.

Other Notes

Fluorescent calcium chelator and indicator with an unusually large shift in fluorescence emission from 480 nm to 400 nm on binding of calcium. Useful for intracellular application

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Sandrine Pouvreau et al.
The Journal of physiology, 567(Pt 3), 815-828 (2005-07-05)
Nitric oxide (NO) generated by skeletal muscle is believed to regulate force production but how this is achieved remains poorly understood. In the present work we tested the effects of NO synthase (NOs) inhibitors on membrane current and intracellular calcium
M K Bijsterbosch et al.
Biochemical and biophysical research communications, 137(1), 500-506 (1986-05-29)
The new Ca2+-probe indo-1 has a high fluorescence intensity, which allows low intracellular dye loadings. Stimulation of indo-1-loaded mouse B cells with anti-Ig antibodies provoked rapid rise of free cytoplasmic Ca2+ from 100 nM to greater than 1 microM, followed
R Madelaine Paredes et al.
Methods (San Diego, Calif.), 46(3), 143-151 (2008-10-22)
Our understanding of the underlying mechanisms of Ca2+ signaling as well as our appreciation for its ubiquitous role in cellular processes has been rapidly advanced, in large part, due to the development of fluorescent Ca2+ indicators. In this chapter, we
K G Lazzari et al.
The Journal of biological chemistry, 261(21), 9710-9713 (1986-07-25)
The activation of human neutrophils by chemotactic peptides evokes a rapid change in membrane potential and an increase in cytoplasmic Ca2+ levels. These events are followed up to a minute later by detectable levels of microbicidal agents formed by the
Microsphere-based real-time calcium sensing.
Rosario M Sánchez-Martín et al.
Angewandte Chemie (International ed. in English), 45(33), 5472-5474 (2006-07-19)
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