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73881

Sigma-Aldrich

Fluo 3-AM

≥90% (TLC)

Synonym(s):
4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4′-methyl-2,2′(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester
Empirical Formula (Hill Notation):
C51H50Cl2N2O23
CAS Number:
Molecular Weight:
1129.85
NACRES:
NA.32

Quality Level

Assay

≥90% (TLC)

form

powder

fluorescence

λex 506 nm; λem 525 nm in 0.1 M Tris pH 8.0, esterase

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc(ccc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(OCOC(C)=O)c(Cl)cc35

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This Item
46393343242-MF0888
Fluo 3-AM ≥90% (TLC)

Sigma-Aldrich

73881

Fluo 3-AM

Fluo-3 suitable for fluorescence, ~70%

Sigma-Aldrich

46393

Fluo-3

FLUO 3/AM Membrane-permeable version of FLUO 3. Unlike all other Ca2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg2+.

Sigma-Aldrich

343242-M

FLUO 3/AM

Fura 2-AM ≥95% (HPLC)

Sigma-Aldrich

F0888

Fura 2-AM

form

powder

form

solid

form

solid

form

powder

fluorescence

λex 506 nm; λem 525 nm in 0.1 M Tris pH 8.0, esterase

fluorescence

λex 506 nm; λem 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca2+

fluorescence

-

fluorescence

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Application

Cell permeable fluorescent indicator of intracellular Ca2+; non-fluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2+; lower binding affinity allows measurement of higher peaks of Ca2+ transients than with Fura 2.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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