Merck
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A3221

Sigma-Aldrich

Iodoacetamide

Single use vial of 56 mg

Linear Formula:
ICH2CONH2
CAS Number:
Molecular Weight:
184.96
Beilstein:
1739080
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.56

Quality Level

assay

≥99% (HPLC)

form

powder

packaging

vial of 56 mg (Single use)

mp

92-95 °C (lit.)

solubility

H2O: soluble 50 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

SMILES string

NC(=O)CI

InChI

1S/C2H4INO/c3-1-2(4)5/h1H2,(H2,4,5)

InChI key

PGLTVOMIXTUURA-UHFFFAOYSA-N

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Application

Iodoacetamide may be used as an alkylating reagent for cysteine residues in peptide sequencing and peptide/protein analytical analysis. It may be used in 2-D protein electrophoresis to reduce streaking and improve resolution by eliminating artifacts that could result from disulfide formation during electrophoresis. By virtue of its reaction with cysteine, iodoacetamide is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibition of ribonuclease. Iodoacetamide may be used in disulfide stress research.

Packaging

10 vials in glass insert
Packaged in sealed ampules

Biochem/physiol Actions

Iodoacetamide acts as an alkylating reagent for cysteine residues in peptide sequencing. It is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibiting ribonuclease.

Legal Information

Technology developed in partnership with Proteome Systems
Proteome Systems is a trademark of Proteome Systems Ltd

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 4 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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