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D8537

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

Dulbecco’s Phosphate Buffered Saline
1 of 6 reviewers received a sample product or took part in a promotion

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DPBS

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100 ML
€14.70
500 ML
€25.30
1 L
€42.50
6 X 500 ML
€149.00
6 X 1 L
€230.00
24 X 500 ML
€505.00

€14.70


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100 ML
€14.70
500 ML
€25.30
1 L
€42.50
6 X 500 ML
€149.00
6 X 1 L
€230.00
24 X 500 ML
€505.00

About This Item

MDL number:
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75

€14.70


Please contact Customer Service for Availability

Request a Bulk Order

Product Name

Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

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This Item
59321C59331C59300C
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

300

Quality Level

400

Quality Level

400

Quality Level

500

form

liquid

form

liquid

form

liquid

form

liquid

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

impurities

endotoxin, tested

impurities

-

impurities

-

impurities

-

d-PBS, Dulbeccos PBS, Dulbeccos Buffer, Phosphate Buffered Saline, buffered saline Dulbeccos, balanced salt solution (BSS), bss for mammalian cells,

Isotonic saline, cell washing solution, cell suspension solution

Isotonic saline, cell washing solution, cell suspension solution

DPBS, Isotonic saline, cell washing solution, cell suspension solution

General description

Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. It provides water and inorganic ions essential for normal cell metabolism. DPBS with calcium and magnesium facilitates cell binding and clumping. Dulbecco′s PBS without these ions can be used to wash and rinse suspended cells. Phosphate buffering maintains the pH in the physiological range.

Application

Dulbecco′s Phosphate Buffered Saline has been used:

  • for the dilution of fresh red blood cells to study the trapping of red blood cell aggregate.[1]
  • to wash the wells of a microtiter plate.[2]
  • to wash the cells before resuspending them in lysis buffer for DNA isolation.[3]
  • as a component of embryo holding medium for vitrification of embryos.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Reviews

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  1. Atlanta, GA
    • Reviews 4
    • Votes 9
    5 out of 5 stars.

    All-purpose DPBS

    It's DPBS with calcium-magnesium modification. Worked pretty well for me for various applications.

    Helpful?

  2. 5 Ratings-Only Reviews

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