H1009
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5 mL | Please contact Customer Service for Availability | €59.60 | |
| 100 mL | Please contact Customer Service for Availability | €68.10 | |
| 500 mL | Please contact Customer Service for Availability | €164.00 |
23.3 mmHg ( 30 °C)
29.0-32.0% (titration)
liquid
~2 yr
reagent type: oxidant
Catalysis
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30 % (w/w) in H2O
cell based assay: suitable, immunohistochemistry: suitable
1.110 g/cm3
suitable for DNA precipitation
general analytical
pharmaceutical
2-8°C
OO
1S/H2O2/c1-2/h1-2H
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This Item | |||
|---|---|---|---|
| concentration 30 % (w/w) in H2O | concentration 30 wt. % in H2O | concentration 30 wt. % in H2O | concentration 50 wt. % in H2O |
| assay 29.0-32.0% (titration) | assay ≤32% | assay 29.0-32.0% (ACS specification) | assay 48-52% H2O2 basis (by KMnO4, titration) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form liquid | form liquid | form liquid | form - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| vapor pressure 23.3 mmHg ( 30 °C) | vapor pressure 23.3 mmHg ( 30 °C) | vapor pressure 23.3 mmHg ( 30 °C) | vapor pressure 23.3 mmHg ( 30 °C) |
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Danger
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5.1B - Oxidizing hazardous materials
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This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.
This procedure may be used for all Catalase products.
| SKU | GTIN |
|---|---|
| H1009-500ML | 04061833663677 |
| H1009-5ML | 04061833663684 |
| H1009-100ML | 04061838255938 |
Yes, this product is recommended. It is the hydrogen peroxide that is used in our Enzymatic Assay of Peroxidase, using Pyrogallol as the substrate. See: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-peroxidase
Please note that since this is a colorimetric assay, the color of the sample may interfere with the absorbance readings. So the soil sample may need to be decolorized before testing for enzymatic activity.
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
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If 0.3% H2O2 (by volume) is to be achieved, it would be necessary to add 0.15 mL of H2O2, which would be 0.5 mL of the 30% solution. If the solution is to be exactly 0.3% H2O2, it would be best to only use 49.5 mL of PBS, to account for the added volume of H2O2 solution. If the 0.3% is to be m/v or v/v, the density would need to be used in order to convert.
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The molarity of this product is calculated to be 9.791 M based on a density of 1.11 g/mL, a formula weight of 34.01 g/mol, and a concentration of 30 w/w%. Here is the calculation:
Molarity = Density x concentration x 1000 / formula weight = 1.11 x 0.3 x 1000 / 34.01 = 9.791 M.
To make a 100 mM solution, use the Dilution Formula: C1V1 = C2V2 to find the volume V1 of the 30% H2O2 solution needed.
C1 = 9.791 M (concentration of the stock solution)
C2 = 0.1 M (desired concentration)
V2 = 0.001 L + V1 (final volume, which is the sum of 1 mL of water and the volume of H2O2 added)
Rearrange the formula to solve for V1:
9.791 x V1 = 0.1 x (0.001 + V1)
V1 = 0.1 x 0.001 / (9.791 - 0.1) = ~0.0000103 L = ~10.3 µL
Therefore, approximately 10.3 µL of this product is required for a 100 mM H2O2 solution when mixed with 1 mL of water.
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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This product contains Dipicolinic acid at 5 – 10ppm as a stabilizer.
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Using a procedure meant for lower concentrations of hydrogen peroxide may lead to less accurate results. Solvay chemicals has a method for determination of the concentration of hydrogen peroxide (20-70%) by titration with potassium permanganate solution: https://www.solvay.com/sites/g/files/srpend221/files/2019-10/H2O2%20Determination%20of%20Hydrogen%20Peroxide%20Concentration%20-20%20to%2070-.pdf
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Unfortunately, there is no verified contact time for the use of product H1009 in disinfecting.
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This product is not assigned an expiration date. It is assigned a recommended retest date of approximately one year after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.
For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
Please see the link below to access a specific lot or to review a sample Certificate:
https://www.sigmaaldrich.com/product/sigma/H1009#product-documentation
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The molarity of this product will change slightly from lot to lot, based on the final concentration. The concentration range is 29 - 32 %. The molarity can be calculated using the basic equation (density in g/L x final percentage)/molecular weight. The molarity of a 30 % solution of this product can be calculated by the following:
(1110 g/L X 0.30) / 34.01 = 9.79 M.
The volume of product required to prepare 7 mL of 0.005 M H2O2 would be 3.6 ul. For ease of pipetting, it may be more practical to prepare a larger volume. For example:
Use 3.6 uL to prepare 7 mL at 5 mM
Use 7.7 uL to prepare 15 mL at 5 mM
Use 10.3 uL to prepare 20 mL at 5 mM
Alternatively, an intermediate working solution can be prepared that would require further dilution.
To prepare a 1 M stock from the 9.79 M concentrate, a dilution may be prepared using the M1V1 =M2V2 formula.
In this case, 1.02 mL of the 9.79 M stock is diluted to 10 mL to achieve a 1 M concentration.
From the 1 M solution dilute 0.035 mL (35 ul) to a final volume of 7 mL to achieve a 5 mM concentration.
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