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Escort III Transfection Reagent

Lipid reagent for transfecting sensitive and primary cells

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for molecular biology

Quality Level


liquid (aqueous solution)


 mL sufficient for 250-1000 transfections


1 mg/mL


transfection: suitable

storage temp.


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General description

Escort III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells.


Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 μl Escort III and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort III:

C2C12 myotubes
Cardiomyocytes (rat)
Fibroblasts (rat)
Germ cells (male rat)
Hepatocytes (rat and hamster)
Keratinocytes (human)
Myoblasts (mouse and quail)
Myocytes (mouse)
Retinal Neurons (rat)
Tracheobronchial cells (sheep)

Features and Benefits

  • Suitable for stable and transient transfection
  • Optimized for a wide variety of primary cells
  • Low toxicity
  • Compatible with both serum and serum-free transfection protocols
  • Ideal for PC-12 cells


Escort III formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort III is confidential.


Do not freeze.


A stable complex is formed when Escort III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.


The product bulletin providin detailed use protocol for easy DNA transfection.

Product manual provides detailed protocol for easy DNA transfection.

Related Content

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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