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Nuclease S1 from Aspergillus oryzae

for single-strand DNA/RNA digestion

Endonuclease S1
CAS Number:
Enzyme Commission number:
MDL number:

biological source

Aspergillus sp. (A. oryzae)




≥100000 units/mL

shipped in

wet ice

storage temp.


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General description

The Nuclease S1 enzyme from Aspergillus oryzae has the ability to degrade single-stranded oligonucleotides composed of either deoxynucleotides or ribonucleotides.


Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA. It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.

Biochem/physiol Actions

SI nuclease from Aspergillus oryzae can generate double-stranded DNA breaks in response to DNA nicks or abasic sites.
Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5′-phosphomononucleotide and 5′-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.

Unit Definition

One unit will cause 1.0 microgram of single-stranded nucleic acid to become perchloric acid soluble per minute at pH 4.6 at 37°C.

Physical form

Solution containing 30 mM sodium acetate, 50 mM NaCl, 1 mM ZnCl2, 50% glycerol, 2 mg/ml protein

Kit Components Only

Product No.

  • 30mM Sodium acetate .25-.25 %

  • 50mM Sodium chloride .29 %

  • 1mM Zinc chloride .01 %

  • Glycerol 50 %

  • 2mg/mL Protein .2 %

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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More documents

Quotes and Ordering

M A Chaudhry et al.
Nucleic acids research, 23(19), 3805-3809 (1995-10-11)
Defined DNA substrates containing discrete abasic sites or paired abasic sites set 1, 3, 5 and 7 bases apart on opposite strands were constructed to examine the reactivity of S1, mung bean and P1 nucleases towards abasic sites. None of
P Beard et al.
Journal of virology, 12(6), 1303-1313 (1973-12-01)
S(1) nuclease, the single-strand specific nuclease from Aspergillus oryzae can cleave both strands of circular covalently closed, superhelical simian virus 40 (SV40) DNA to generate unit length linear duplex molecules with intact single strands. But circular, covalently closed, nonsuperhelical DNA
S1 nuclease from Aspergillus oryzae for the detection of DNA damage and repair in the gamma-irradiated intracerebral rat gliosarcoma 9L.
P H Gutin et al.
Radiation research, 72(1), 100-106 (1977-10-01)
T E Shenk et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(3), 989-993 (1975-03-01)
S1 nuclease (EC 3.1.4.X), a single-strand-specific nuclease, can be used to accurately map the location of mutational alterations in simian virus 40 (SV40) DNA. Deletions of between 32 and 190 base pairs, which are at or below the limit of
Xinjian Yang et al.
Chemical communications (Cambridge, England), 47(28), 8133-8135 (2011-06-02)
We have demonstrated a DNA-templated small molecule ensemble that allows the label-free fluorescence turn-on detection of enzymatic or oxidative cleavage of single-stranded DNA with high sensitivity and selectivity.


Enzymatic Assay of Nuclease S1

Enzymatic Assay of Nuclease S1

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