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Supelco

SP®-2380 Capillary GC Column

L × I.D. 30 m × 0.53 mm, df 0.20 μm

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UNSPSC Code:
41115710

material

fused silica

Quality Level

100

Agency

meets requirements for USP G48

parameter

≤25-275 °C temperature (isothermal or programmed)

Beta value

663

df

0.20 μm

technique(s)

gas chromatography (GC): suitable

L × I.D.

30 m × 0.53 mm

matrix active group

Stabilized; poly(90% biscyanopropyl/10% cyanopropylphenyl siloxane) phase

application(s)

agriculture
chemicals and industrial polymers
clinical
food and beverages

column type

capillary highly polar

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General description

Capillary GC column is also known as open tubular column. The carrier gas flows through the central aperture and is unrestricted throughout the length of the column.
Application: A highly polar cyanosiloxane column commonly used for separation of geometric (cis/trans) fatty acid methyl ester (FAME) isomers as a group. Also useful when a highly polar general purpose column with good thermal stability is required.
USP Code: This column meets USP G48 requirements.
Phase:
  • Stabilized
  • Poly(90% biscyanopropyl/10% cyanopropylphenyl siloxane)
Temp. Limits:
  • Subambient to 275 °C (isothermal or programmed)

Application

  • SP®-2380 Capillary Column may be used for determination of fatty acid composition in crude oil in capillary gas chromatography, used along with normal and reverse-phase high-performance liquid chromatography (HPLC) in order to study the content and composition of tocopherols, sterols, and phospholipids in soybean oils derived from genetically-modified soybeans.
  • It was found suitable to be used for the identification of fatty acid methyl esters from mammalian samples by fast gas chromatography.
  • It was suitable for determining the hydrogenation level and the level of isomerisation development during the hydrogenation process of edible oils by capillary gas chromatography.
  • It was also suitable to be used in gas chromatography equipped with flame ionization detector (FID) for analysing the cytotoxicity of fatty acids from seed oils containing conjugated linolenic acids (CLN) on mouse tumor and human monocytic leukemia cells.

Other Notes

We offer a variety of chromatography accessories including analytical syringes

Legal Information

SP is a registered trademark of Sigma-Aldrich Co. LLC

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effect of genetic modification on the content and composition of bioactive constituents in soybean oil
Journal of the American Oil Chemists' Society, 73 (5), 581-586 (1996)
R Suzuki et al.
Lipids, 36(5), 477-482 (2001-07-04)
The cytotoxicity of fatty acids from seed oils containing conjugated linolenic acids (CLN) was studied. Fatty acids from pomegranate, tung, and catalpa were cytotoxic to human monocytic leukemia cells at concentrations exceeding 5 microM for pomegranate and tung and 10
Fast gas chromatography for the identification of fatty acid methyl esters from mammalian samples.
Stark K and Salem N.
Liposome Technology, 17 (8), 181-181 (2005)
E M Bondia et al.
Journal of chromatography. B, Biomedical applications, 658(2), 369-374 (1994-08-19)
Total fatty acids in plasma of neonates have been analysed as their methyl esters by gas chromatography. They were separated on a capillary column coated with a SP-2380 stationary phase. As little as 100 microliters of plasma is used for
Use of capillary gas chromatography for determining the hydrogenation level of edible oils.
Mateja N and Smidovnik A.
Journal of Chromatography A, 767 (1), 335-339 (1997)
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