568523-U
Discovery® HS C18 (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
Synonym(s):
Discovery RP18 HPLC Column
About This Item
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product name
Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L1
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
20% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
surface area
300 m2/g
surface coverage
3.2 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical particle platform
fully porous particle
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
120 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
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General description
Features and Benefits
- Stable, low bleed for LC-MS applications
- Scalable from analytical to preparatory
- Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility
- Higher hydrophobicity for better resolution of difficult analytes
Legal Information
Application
guard cartridge
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Discovery® C18 column aids in HPLC method development for quantifying ephedrine and pseudoephedrine in traditional Chinese medicine.
Extraction and analyses of chlorogenic acid and baicalin active ingredients in traditional Chinese medicine (TCM) according to Chinese pharmacopeia monograph by HPLC with Discovery® C18 column to check: chromatographic data, linearity, specificity and repeatability, LOQ and LOD
Analyze Shuanghuang Lian oral liquid using Discovery® HS C18 HPLC column per Chinese Pharmacopeia 2020. Quantify baicalin, chlorogenic acid, and forsythin with high accuracy.
Analyze Xiaoer Chiqiao Qingre granules for geniposide and forsythin using Discovery® HS C18 column and Supelclean™ LC-Alumina-N SPE.
Related Content
HPLC DiscoveryBIO HS C18 products. Available in 3µm, 5µm and 10µm particle sizes.
Chromatograms
application for HPLCapplication for HPLCapplication for HPLCapplication for HPLCOur team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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