577101-U

Ascentis^® Express 160 Å C30, 2.7 μm HPLC Column

column L × I.D. 10 cm × 2.1 mm

• Synonym(s): Ascentis^® Express 160 Å C30, 2.7 μm HPLC Column

Properties

• material: stainless steel column
• Agency: suitable for USP L62
• product line: Ascentis^®
• feature: endcapped
• extent of labeling: 4.5% Carbon loading
• technique(s): HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable
• column L × I.D.: 10 cm × 2.1 mm
• matrix: Fused-Core^® particle platform; superficially porous particle
• matrix active group: C30 (triacontyldimethyl) bonding phase
• particle size: 2.7 μm
• pore size: 160 Å pore size
• surface area: 90 m²/g
• operating pH range: 2-9
• separation technique: reversed phase

Description

General description

Ascentis^® Express 160 Å C30 is a high-speed, high-performance liquid chromatography column based on the wider-pore 160 Å Fused-Core^® particle design to provide adequate spacing for the long-chain C30 ligands. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 μm thick porous shell and the small overall particle size of 2.7 μm. The endcapped triacontylsilane stationary phase of the C30 provides a stable, reversed-phase packing that can be used for hydrophobic, long-chain, structural isomers such as vitamins and lipids.

Application

UHPLC Analysis of Vitamin A and Vitamin E on Ascentis^® Express C30
UHPLC/MS Analysis of Corn Oil on Ascentis^® Express C30 Compared to Ascentis^® Express C18
UHPLC Analysis of Vitamin K1 on Ascentis^® Express C30
HPLC Analysis of Tocopherols on Ascentis^® Express C30, 2.7 μm

Analysis Note

The target analytes include hydrophobic analytes, lipids, carotenoids, and fat soluable vitamins (A, D, E, K).

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.