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Christian Schmidt et al.
Analytical and bioanalytical chemistry, 406(21), 5171-5185 (2014-03-22)
Toxic organophosphorus compounds (e.g., pesticides and nerve agents) are known to react with nucleophilic side chains of different amino acids (phosphylation), thus forming adducts with endogenous proteins. Most often binding to serine, tyrosine, or threonine residues is described as being
Pete Gagnon et al.
Journal of chromatography. A, 1374, 145-155 (2014-12-08)
Chromatin heteroaggregates comprising nucleosomal arrays, DNA, histone and non-histone host cell proteins contributed 28% of the host contaminant mass in an IgG cell culture harvest. A subset comprising soluble particles of 50-400nm was retained through its histone component by an
Pieter Glibert et al.
International journal of molecular sciences, 15(6), 9407-9421 (2014-05-30)
Proteome studies on hematological malignancies contribute to the understanding of the disease mechanism and to the identification of new biomarker candidates. With the isobaric tag for relative and absolute quantitation (iTRAQ) method we analyzed the protein expression between B-cells of
Manuel Jiménez Aguilar
Journal of hazardous materials, 213-214, 502-504 (2012-03-01)
The aim of this work was to investigate the effect of urine on the absorption of greenhouse gases such as CO(2). Human urine diluted with olive-oil-mill wastewaters (OMW) could be used to capture CO(2) from flue gas of coal-fired power
Carlos Eduardo Braga de Abreu et al.
Plant cell reports, 33(8), 1289-1306 (2014-04-29)
Cowpea cultivars differing in salt tolerance reveal differences in protein profiles and adopt different strategies to overcome salt stress. Salt-tolerant cultivar shows induction of proteins related to photosynthesis and energy metabolism. Salinity is a major abiotic stress affecting plant cultivation
Yuanzhong Yang et al.
Analytica chimica acta, 872, 84-94 (2015-04-22)
In this study, the advantages of carrying out the analysis of peptides and tryptic digests of proteins under gradient elution conditions at pH 6.5 by reversed-phase liquid chromatography (RP-HPLC) and in-line electrospray ionisation mass spectrometry (ESI-MS) are documented. For these
David R Barnidge et al.
Journal of proteome research, 13(11), 5198-5205 (2014-08-20)
We previously described a microLC-ESI-Q-TOF MS method for identifying monoclonal immunoglobulins in serum and then tracking them over time using their accurate molecular mass. Here we demonstrate how the same methodology can be used to identify and characterize polyclonal immunoglobulins
Zhoufang Zeng et al.
Analytical chemistry, 84(5), 2118-2123 (2012-02-14)
In this paper, a new technique has been proposed to achieve simultaneous peptides/proteins enrichment and wash-free self-desalting on a novel sample support with a circle hydrophobic-hydrophilic-hydrophobic pattern. Upon deposition, the sample solution is first concentrated in a small area by
Yoshiro Saito et al.
Journal of neuropathology and experimental neurology, 73(7), 714-728 (2014-06-12)
DJ-1, the product of a causative gene of a familial form of Parkinson disease, undergoes preferential oxidation of Cys106 (cysteine residue at position 106) under oxidative stress. Using specific monoclonal antibodies against Cys106 oxidized DJ-1 (oxDJ-1), we examined oxDJ-1 immunoreactivity
Jean Dubayle et al.
Vaccine, 33(11), 1360-1368 (2015-02-11)
Recently, several virus studies have shown that protein glycosylation play a fundamental role in the virus-host cell interaction. Glycosylation characterization of the envelope proteins in both insect and mammalian cell-derived dengue virus (DENV) has established that two potential glycosylation residues
Qi Chen et al.
Analytical and bioanalytical chemistry, 407(3), 1027-1032 (2014-10-20)
To analyze the proteome of an extremely low number of cells or even a single cell, we established a new method of digesting whole cells into mass-spectrometry-identifiable peptides in a single step within 2 h. Our sampling method greatly simplified the
Yanjie Li et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 966, 147-153 (2014-05-27)
Lung cancer is currently the leading cause of cancer-related mortality worldwide. It is, therefore, important to enhance understanding and add a new auxiliary detection tool of lung cancer. In this work, serum metabolic characteristics of lung cancer were investigated with
Martyna Kielmas et al.
Analytical and bioanalytical chemistry, 407(9), 2557-2567 (2015-02-01)
Glycation of α-crystallin is responsible for age- and diabetic-related cataracts, which are the main cause of blindness worldwide. We optimized the method of identification of lysine residues prone to glycation using the combination of LC-MS, isotopic labeling, and modified synthetic
Laura Botto et al.
PloS one, 9(5), e98344-e98344 (2014-05-27)
The prion protein (PrPC) is highly expressed within the nervous system. Similar to other GPI-anchored proteins, PrPC is found in lipid rafts, membrane domains enriched in cholesterol and sphingolipids. PrPC raft association, together with raft lipid composition, appears essential for
Mustafa Tugrul Masatcioglu et al.
Journal of the science of food and agriculture, 94(12), 2562-2568 (2014-02-06)
Acrylamide is a possible carcinogen and known to form in heat-treated carbohydrate-rich foods. This study was designed to investigate the effects of different ingredients (reducing sugars, chemical leavening agents, citric acid), processing conditions (feed moisture content: 22, 24 or 26%
Marina Makrecka et al.
Molecular and cellular biochemistry, 395(1-2), 1-10 (2014-06-01)
In the heart, a nutritional state (fed or fasted) is characterized by a unique energy metabolism pattern determined by the availability of substrates. Increased availability of acylcarnitines has been associated with decreased glucose utilization; however, the effects of long-chain acylcarnitines
Juan Li et al.
Virology, 478, 86-98 (2015-03-03)
Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins. Glycans of major envelope glycoprotein 5 (GP5) are proposed as important for virus assembly and entry into permissive cells.
Ida Herdlevaer et al.
Annals of clinical and translational neurology, 7(11), 2231-2242 (2020-10-04)
Identify the subcellular location and potential binding partners of two cerebellar degeneration-related proteins, CDR2L and CDR2, associated with anti-Yo-mediated paraneoplastic cerebellar degeneration. Cancer cells, rat Purkinje neuron cultures, and human cerebellar sections were exposed to cerebrospinal fluid and serum from
J Xu et al.
Cell death & disease, 6, e1616-e1616 (2015-01-23)
The selenoprotein thioredoxin reductase 1 (TrxR1) has several key roles in cellular redox systems and reductive pathways. Here we discovered that an evolutionarily conserved and surface-exposed tryptophan residue of the enzyme (Trp114) is excessively reactive to oxidation and exerts regulatory
Yuhong Liu et al.
Journal of chromatography. A, 1364, 64-73 (2014-09-04)
Methicillin resistant Staphylococcus aureus (MRSA) is a bacterium that is resistant to many antibiotics. Resistance to methicillin is related to production of penicillin binding protein 2a (PBP2a). The currently presented research involves the development of antibody-linked immunoaffinity solid phase microextraction
Veluchamy A Barathi et al.
Journal of proteome research, 13(11), 4647-4658 (2014-09-12)
Atropine, a muscarinic antagonist, is known to inhibit myopia progression in several animal models and humans. However, the mode of action is not established yet. In this study, we compared quantitative iTRAQ proteomic analysis in the retinas collected from control
Gamal Wareth et al.
Biochemical and biophysical research communications, 456(1), 202-206 (2014-12-03)
Brucella (B.) species lack classical virulence factors, but escape effectively the immune response of the host. The species Brucella abortus and Brucella melitensis infect predominantly cattle and small ruminants such as sheep or goats, respectively, but account also for most
Nicholas W Bateman et al.
Journal of proteome research, 14(4), 1900-1910 (2015-03-10)
A majority of high-grade (HG) serous ovarian cancer (SOC) patients develop resistant disease despite high initial response rates to platinum/paclitaxel-based chemotherapy. We identified shed/secreted proteins in preclinical models of paclitaxel-resistant human HGSOC models and correlated these candidate proteins with patient
Caterina Pipino et al.
Stem cells and development, 23(24), 2959-2971 (2014-07-19)
Human amniotic fluid mesenchymal stem cells (hAFMSCs) are promising for therapeutic applications in bone damage. Calcium sensing receptor (CaSR), a G protein-coupled receptor, plays a physiological role in the regulation of bone metabolism. Thus, the bone CaSR could be targeted
Jonathan Ashby et al.
ACS applied materials & interfaces, 6(17), 15412-15419 (2014-08-22)
Nanoparticles (NPs) adsorb proteins when in the biological matrix, and the resulted protein corona could affect NP-cell interactions. The corona has a dynamic nature with the adsorbed proteins constantly exchanging with the free proteins in the matrix at various rates.
Sharon D'Souza et al.
Investigative ophthalmology & visual science, 55(6), 3396-3402 (2014-05-08)
New femtosecond laser platforms may reduce ocular surface interference and LASIK-associated dry eye. This study investigated tear protein profiles in subjects who underwent LASIK using two femtosecond lasers to assess differences in protein expression. This was a randomized interventional clinical
Laura Sánchez-Hernández et al.
Journal of chromatography. A, 1359, 317-324 (2014-08-03)
This paper represents the first report of a capillary electrophoresis (CE) method compatible with mass spectrometry (MS) detection for simultaneously analyzing seven neonicotinoid insecticides (acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid and thiamethoxam). Different variables affecting CE separation (buffer concentration, pH
T T Nha Tran et al.
Rapid communications in mass spectrometry : RCM, 29(2), 182-190 (2015-02-03)
To use negative-ion nano-electrospray ionization mass spectrometry of peptides from the tryptic digest of ricin D, to provide sequence information; in particular, to identify disulfide position and connectivity. Negative-ion fragmentations of peptides from the tryptic digest of ricin D was
Lusia Sepiashvili et al.
Molecular & cellular proteomics : MCP, 13(12), 3572-3584 (2014-10-02)
HPV-positive oropharyngeal carcinoma (OPC) patients have superior outcomes relative to HPV-negative patients, but the underlying mechanisms remain poorly understood. We conducted a proteomic investigation of HPV-positive (n = 27) and HPV-negative (n = 26) formalin-fixed paraffin-embedded OPC biopsies to acquire
Dinh-Duy Pham et al.
International journal of pharmaceutics, 454(2), 668-677 (2013-04-23)
We have designed a novel formulation of pyrazinamide (PZA), an antitubercular drug within large porous particles intended for deep lung delivery. By simply spray-drying PZA, we have obtained crystalline particles of the δ polymorph of PZA that were unstable and
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