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Shewit Kalayou et al.
Cell biology and toxicology, 30(6), 361-376 (2014-11-25)
The mycotoxin alternariol (AOH) is an important contaminant of fruits and cereal products. The current study sought to address the effect of a non-toxic AOH concentration on the proteome of the steroidogenic H295R cell model. Quantitative proteomics based on stable
Victoria Pando-Robles et al.
Journal of proteomics, 111, 16-29 (2014-07-11)
Dengue is an important and growing public health problem worldwide with an estimated 100million new clinical cases annually. Currently, no licensed drug or vaccine is available. During natural infection in humans, liver cells constitute one of the main targets of
C Gröer et al.
Journal of pharmaceutical and biomedical analysis, 100, 393-401 (2014-09-15)
Cytochrome P450 (CYP) enzymes and UDP-glucuronosyltransferases (UGT) are major determinants in the pharmacokinetics of most drugs on the market. To investigate their impact on intestinal and hepatic drug metabolism, we developed and validated quantification methods for nine CYP (CYP1A2, CYP2B6
Nida Zaidi et al.
The journal of physical chemistry. B, 118(46), 13025-13036 (2014-10-23)
Sodium dodecyl sulfate (SDS)-glycoprotein interaction serves as a model for a biological membrane. To get mechanistic insight into the interaction of SDS and glycoprotein, the effect of SDS on bovine serum fetuin (BSF) was studied in subcritical micellar concentrations at
Hongbing Shao et al.
Nucleic acids research, 42(11), 7104-7112 (2014-05-16)
Genetic and biochemical studies have previously implicated exonuclease 1 (Exo1) in yeast and mammalian mismatch repair, with results suggesting that function of the protein in the reaction depends on both its hydrolytic activity and its ability to interact with other
Vincent Delorme et al.
Biochimie, 107 Pt A, 124-134 (2014-07-30)
A synthetic phosphonate inhibitor designed for lipase inhibition but displaying a broader range of activity was covalently immobilized on a solid support to generate a function-directed tool targeting serine hydrolases. To achieve this goal, straightforward and reliable analytical techniques were
Sergio Alonso-Orgaz et al.
Journal of proteomics, 109, 368-381 (2014-07-30)
Acute myocardial infarction with ST-segment elevation (STEMI) initiates with intraluminal thrombosis and results in total occlusion of the coronary artery. To date, characterization of the coronary thrombus proteome in STEMI patients has not been yet accomplished. Therefore, we aimed to
Francesca Mateo et al.
Molecular cancer, 13, 237-237 (2014-10-22)
Tumor cell subpopulations can either compete with each other for nutrients and physical space within the tumor niche, or co-operate for enhanced survival, or replicative or metastatic capacities. Recently, we have described co-operative interactions between two clonal subpopulations derived from
Elspeth M Beauchamp et al.
Molecular cancer therapeutics, 14(1), 202-212 (2014-10-26)
Arsenic trioxide (As2O3) exhibits potent antineoplastic effects and is used extensively in clinical oncology for the treatment of a subset of patients with acute myeloid leukemia (AML). Although As2O3 is known to regulate activation of several signaling cascades, the key
Abby Sewell et al.
Molecular vision, 20, 1182-1191 (2014-09-16)
To compare the proteomic profile of a clinical isolate of Pseudomonas aeruginosa (P. aeruginosa) obtained from an infected cornea of a contact lens wearer and the laboratory strain P. aeruginosa ATCC 10145. Antibiotic sensitivity, motility, biofilm formation, and virulence tests
Zhiwei Zhu et al.
Eukaryotic cell, 14(3), 252-264 (2015-01-13)
Lipid droplets (LDs) are ubiquitous organelles that serve as a neutral lipid reservoir and a hub for lipid metabolism. Manipulating LD formation, evolution, and mobilization in oleaginous species may lead to the production of fatty acid-derived biofuels and chemicals. However
Yilu Bao et al.
Journal of microbiology and biotechnology, 22(7), 967-974 (2012-05-15)
Cultivation of Spirulina platensis using ammonium salts or wastewater containing ammonium as alternative nitrogen sources is considered as a commercial way to reduce the production cost. In this research, by analyzing the relationship between biomass production and ammonium- N consumption
Paula M Ladwig et al.
Clinical chemistry, 60(8), 1080-1088 (2014-05-07)
Measurement of IgG subclasses is a useful tool for investigation of humoral immune deficiency in the presence of total IgG within reference intervals and IgG4-related disease. Nephelometry has been the method of choice for quantification. We describe an LC-MS/MS method
Kasra Hassani et al.
PloS one, 9(4), e95007-e95007 (2014-04-17)
Protozoan parasites of Leishmania genus are able to successfully infect their host macrophage due to multiple virulence strategies that result in its deactivation. Recent studies suggest Leishmania GP63 to be a critical virulence factor in modulation of many macrophage molecules
Qianying Sheng et al.
Journal of chromatography. A, 1345, 57-67 (2014-05-06)
A-saccharide-based stationary phase for hydrophilic interaction liquid chromatography (HILIC) is presented. The method uses carbonyl di-imidazole (CDI) as a cross-linker for dextran in aqueous solution. Different from the traditional immobilized saccharide stationary phase, this method was using a simple way
Cheng-Kang Chiang et al.
PLoS genetics, 10(10), e1004695-e1004695 (2014-10-21)
The suprachiasmatic nucleus (SCN) acts as the central clock to coordinate circadian oscillations in mammalian behavior, physiology and gene expression. Despite our knowledge of the circadian transcriptome of the SCN, how it impacts genome-wide protein expression is not well understood.
Vadim Demichev et al.
Nature methods, 17(1), 41-44 (2019-11-27)
We present an easy-to-use integrated software suite, DIA-NN, that exploits deep neural networks and new quantification and signal correction strategies for the processing of data-independent acquisition (DIA) proteomics experiments. DIA-NN improves the identification and quantification performance in conventional DIA proteomic
Sachin Kumar et al.
Nanoscale, 7(5), 2023-2033 (2015-01-03)
The objective of this work was to prepare hybrid nanoparticles of graphene sheets decorated with strontium metallic nanoparticles and demonstrate their advantages in bone tissue engineering. Strontium-decorated reduced graphene oxide (RGO_Sr) hybrid nanoparticles were synthesized by the facile reduction of
Ida Herdlevaer et al.
Annals of clinical and translational neurology, 7(11), 2231-2242 (2020-10-04)
Identify the subcellular location and potential binding partners of two cerebellar degeneration-related proteins, CDR2L and CDR2, associated with anti-Yo-mediated paraneoplastic cerebellar degeneration. Cancer cells, rat Purkinje neuron cultures, and human cerebellar sections were exposed to cerebrospinal fluid and serum from
J Xu et al.
Cell death & disease, 6, e1616-e1616 (2015-01-23)
The selenoprotein thioredoxin reductase 1 (TrxR1) has several key roles in cellular redox systems and reductive pathways. Here we discovered that an evolutionarily conserved and surface-exposed tryptophan residue of the enzyme (Trp114) is excessively reactive to oxidation and exerts regulatory
Christopher Schmidt et al.
Journal of bacteriology, 196(19), 3461-3471 (2014-07-23)
The zinc importer ZupT is required for the efficient allocation of zinc to zinc-dependent proteins in the metal-resistant bacterium Cupriavidus metallidurans but not for zinc import per se. The expression of zupT is upregulated under conditions of zinc starvation. C.
Esther Roselló-Lletí et al.
PloS one, 9(11), e112971-e112971 (2014-11-15)
Dilated cardiomyopathy (DCM) is a public health problem with no available curative treatment, and mitochondrial dysfunction plays a critical role in its development. The present study is the first to analyze the mitochondrial proteome in cardiac tissue of patients with
Kun Peng et al.
mAbs, 6(6), 1500-1508 (2014-12-09)
RG7652 is a human immunoglobulin 1 (IgG1) monoclonal antibody (mAb) targeting proprotein convertase subtilisin/kexin type 9 (PCSK9) and is designed for the treatment of hypercholesterolemia. A target-binding enzyme-linked immunosorbent assay (ELISA) was developed to measure RG7652 levels in human serum
Cosima Damiana Calvano et al.
Analytical and bioanalytical chemistry, 407(3), 1015-1022 (2014-12-01)
Direct on-target plate processing of small (ca. 100 μg) fragments of paint samples for MALDI-MS identification of lipid- and protein-based binders is described. Fragments were fixed on a conventional stainless steel target plate by colloidal graphite followed by in situ fast
Neda Saraygord-Afshari et al.
Biotechnology and applied biochemistry, 61(6), 660-667 (2014-03-01)
Patterns obtained in two-dimensional gel electrophoresis (2-DE) in the previously published articles suggest a varying number of proteins. To seek the cause of this variation, we investigated the effect of reduction power on the overall tear proteome maps. To this
Stephanie L Sarbanes et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(1) (2021-01-15)
The journey from plasma membrane to nuclear pore is a critical step in the lifecycle of DNA viruses, many of which must successfully deposit their genomes into the nucleus for replication. Viral capsids navigate this vast distance through the coordinated
Ivana Milic et al.
Journal of mass spectrometry : JMS, 50(3), 603-612 (2015-03-25)
Highly reactive lipid peroxidation-derived carbonyls (oxoLPP) modify protein nucleophiles via Michael addition or Schiff base formation. Once formed, Michael adducts can be further stabilized via cyclic hemiacetals with or without loss of water. Depending on the mechanism of their formation
Marcia R Mauk et al.
The Journal of biological chemistry, 285(27), 20499-20506 (2010-05-01)
The stability of the hemopexin-heme (Hx-heme) complex to dissociation of the heme prosthetic group has been examined in bicarbonate buffers in the presence and absence of various divalent metal ions. In NH(4)HCO(3) buffer (pH 7.4, 20 mm, 25 degrees C)
Aven Lee et al.
American journal of physiology. Renal physiology, 308(7), F784-F792 (2015-01-15)
ClC-5 is a chloride/proton exchanger that plays an obligate role in albumin uptake by the renal proximal tubule. ClC-5 forms an endocytic complex with the albumin receptor megalin/cubilin. We have identified a novel ClC-5 binding partner, cytosolic aspartyl aminopeptidase (DNPEP;
Yu Chang Kim et al.
Environmental science & technology, 45(18), 7737-7745 (2011-08-17)
This paper analyzes the structural features of a spiral-wound forward-osmosis (SW FO) membrane module via an experimental approach and presents the relationships between the water flux and operating conditions for design and operation of a large-scale FO process. The SW
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