amino acids 158-178.
IMMUNOGEN: Recombinant protein from rat myogenin.
ISOTYPE: IgG1
APPLICATIONS: Western blot: 1 μg/mL. Reacts with the ~34 kD protein.
Immunohistochemistry: 1-2 μg/mL on
Storage 2-8°C; After opening, transfer contents to
small vial provided for your convenience.
Mattson Galaxy 3020 FT-IR
70 eV Electron Ionization Mass Spectrum
40 60 80 100 120 140 160 180 200 220
605-612.
Chen, F. et al., Cancer Immunology Immunotherapy (1991) 33:158-164.
Chen, F. et al., J National Cancer Institute (1991) 83:200-204.
Chen, F. et al., FASEB J. (1990) 4:3033-3039.
Chen, F. et al
densitometry)
Figure 2.
Specific Activity of Typical Lot
116–158 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5 mM
glycerol 2-phosphate, 25 mM MgCl2
Immunogen: Recombinant protein TNF-alpha analog
Immunogen Sequence: GI # 18152208, sequence 1 - 158
The product is a clear, colorless solution in phosphate
buffered saline, pH 7.2, containing 0.02%
maximum wavelength 387 nm
is 26,300 (pH 2-8). The difference in molar extinction
coefficients between oxidized and reduced PMS is
25,000.1
Melting Point: 158-160 °C
PMS is an electron acceptor and
Specific Activity: 200 units/g solid
Unit Definition: One unit will liberate 1.0 mg of
N-acetyl-D-glucosamine from chitin per hour at pH 6.0
at 25 C in a 2-hour assay.
Precautions
supplied as 200 µg/ml in 10 mM
phosphate buffered saline (PBS), pH 7.4, containing
0.2% bovine serum albumin (BSA) and 0.09% sodium
azide.
Storage/Stability
For continuous use, store at 2-8 °C for up
Bergmeyer,
H.U. ed.) 3rd ed., Volume II, 157-158, Verlag Chemie, Deerfield Beach, FL
NOTES:
1. This assay is based on the cited reference.
2. Where Sigma Product or Stock
does not affect axonal caliber or the
transit of cargoes in slow axonal transport., J. Cell
Biol., 158, 681-693 (2002).
4. Rao, M.V., et al., The neurofilament middle
molecular mass subunit
CD4+ T cells apoptosis: a possible mechanism of bystander cell death in HIV infection. J.
Immunol. 158(5):2456-63.
Sieg, S., et al. (1997) Fas ligand deficiency in HIV disease. Proc. Nat’l. Acad. Sci
the glycine modulatory site of the
NMDA receptor expressed in Xenopus oocytes Brain Res. 510, 158-160 (1990)
9. Flood, J.F., et al., Eur. J. Pharmacol., 221, 249 (1992).
10. Schuster, G.M. and Schmidt
Guidebook to Cytokines and Their
Receptors, Nicola, N., ed., Oxford Press (New
York, NY: 1994), pp. 158-160.
2. Murakami, H., and Nagata, S., Granulocyte colony
stimulating factor, in The Cytokine Handbood
Guidebook to Cytokines and Their
Receptors, Nicola, N., ed., Oxford Press (New
York, NY: 1994), pp. 158-160.
2. Murakami, H., and Nagata, S., Granulocyte colony
stimulating factor, in The Cytokine Handbood
from 0.1-200 µg/ml) is transferred to the
lower compartment of a 96 well chemotaxis chamber.
The chemotaxis chamber is then assembled using a
PVP-free polycarbonate filter (5 micron size) and
2 x 106
Science,
266, 1395-1399 (1994).
2. Hedrick, J.A., et al., Lymphotactin is produced by
NK cells and attracts both NK cells and T cells in
vivo. J. Immunol., 158, 1533-1540 (1997).
3. Wang, J.D.
Guidebook to Cytokines and Their
Receptors, Nicola, N., ed., Oxford Press (New
York, NY: 1994), pp. 158-160.
2. Murakami, H., and Nagata, S., Granulocyte colony
stimulating factor, in The Cytokine Handbook
Guidebook to Cytokines and Their
Receptors, Nicola, N., ed., Oxford Press (New
York, NY: 1994), pp. 158-160.
2. Murakami, H., and Nagata, S., Granulocyte colony
stimulating factor, in The Cytokine Handbood