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Pramod Kumar et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(3), 1788-1798 (2020-01-09)
The lipid dependence of the nicotinic acetylcholine receptor from the Torpedo electric organ has long been recognized, and one of the most consistent experimental observations is that, when reconstituted in membranes formed by zwitterionic phospholipids alone, exposure to agonist fails
Guangtao Li et al.
Journal of lipid research, 61(5), 758-766 (2020-01-23)
The formation and properties of liquid-ordered (Lo) lipid domains (rafts) in the plasma membrane are still poorly understood. This limits our ability to manipulate ordered lipid domain-dependent biological functions. Giant plasma membrane vesicles (GPMVs) undergo large-scale phase separations into coexisting
Max Piffoux et al.
ACS nano, 12(7), 6830-6842 (2018-07-06)
Extracellular vesicles (EVs) are recognized as nature's own carriers to transport macromolecules throughout the body. Hijacking this endogenous communication system represents an attractive strategy for advanced drug delivery. However, efficient and reproducible loading of EVs with therapeutic or imaging agents
Kari Kusler et al.
The journal of physical chemistry. B, 120(50), 12883-12889 (2016-12-14)
Phosphatidylserine (PS) has previously been found to bind Cu2+ in a ratio of 1 Cu2+ ion per 2 PS lipids to form a complex with an apparent dissociation constant that can be as low as picomolar. While the affinity of
Yasunori Watanabe et al.
Nature communications, 6, 7922-7922 (2015-08-04)
Eukaryotic cells are compartmentalized into membrane-bounded organelles whose functions rely on lipid trafficking to achieve membrane-specific compositions of lipids. Here we focused on the Ups1-Mdm35 system, which mediates phosphatidic acid (PA) transfer between the outer and inner mitochondrial membranes, and
Liposome Disruption Assay to Examine Lytic Properties of Biomolecules.
Jimah JR, et al.
Bio-protocol, 7(15) (2017)
Sarah N Sipe et al.
Analytical chemistry, 92(1), 899-907 (2019-11-26)
Development of chemical chaperones to solubilize membrane protein complexes in aqueous solutions has allowed for gas-phase analysis of their native-like assemblies, including rapid evaluation of stability and interacting partners. Characterization of protein primary sequence, however, has thus far been limited.
Kelly E Burns et al.
Scientific reports, 6, 28465-28465 (2016-06-24)
Positively charged antimicrobial peptides have become promising agents for the treatment of cancer by inducing apoptosis though their preferential binding and disruption of negatively charged membranes, such as the mitochondrial membrane. (KLAKLAK)2 is such a peptide but due to its
Nicole Harmouche et al.
Biophysical journal, 115(6), 1033-1044 (2018-09-10)
A synergistic enhancement of activities has been described for the amphipathic cationic antimicrobial peptides magainin 2 and PGLa when tested in antimicrobial assays or in biophysical experiments using model membranes. In the presence of magainin 2, PGLa changes from an
Zvonimir Boban et al.
Cell biochemistry and biophysics, 78(2), 157-164 (2020-04-23)
Giant unilamellar vesicles (GUVs) are used extensively as models that mimic cell membranes. The cholesterol (Chol) content in the fiber cell plasma membranes of the eye lens is extremely high, exceeding the solubility threshold in the lenses of old humans.
Charles G Cranfield et al.
Langmuir : the ACS journal of surfaces and colloids, 33(26), 6630-6637 (2017-06-14)
Cyclotides are cyclic disulfide-rich peptides that are chemically and thermally stable and possess pharmaceutical and insecticidal properties. The activities reported for cyclotides correlate with their ability to target phosphatidylethanolamine (PE)-phospholipids and disrupt cell membranes. However, the mechanism by which this
Yong-Guang Gao et al.
Analytical chemistry, 92(4), 3417-3425 (2020-01-24)
In vitro assessment of lipid intermembrane transfer activity by cellular proteins typically involves measurement of either radiolabeled or fluorescently labeled lipid trafficking between vesicle model membranes. Use of bilayer vesicles in lipid transfer assays usually comes with inherent challenges because
Alberto Ceccon et al.
The journal of physical chemistry. B (2018-08-30)
Dark state exchange saturation transfer (DEST) and lifetime line-broadening (Δ R2, the difference in the measured transverse relaxation rates for the observable species in the presence and absence of exchange with a species characterized by very large intrinsic transverse relaxation
Sónia Troeira Henriques et al.
Journal of the American Chemical Society, 141(51), 20460-20469 (2019-11-26)
Peptides with pharmaceutical activities are attractive drug leads, and knowledge of their mode-of-action is essential for translation into the clinic. Comparison of native and enantiomeric peptides has long been used as a powerful approach to discriminate membrane- or receptor-mediated modes-of-action
Arnaud Marquette et al.
Biochimica et biophysica acta. Biomembranes, 1862(2), 183149-183149 (2019-12-10)
Viral protein R (Vpr) is a small accessory protein of 96 amino acids that is present in Human and simian immunodeficiency viruses. Among the very different properties that Vpr possesses we can find cell penetrating capabilities. Based on this and
Nicholas M Frame et al.
Journal of molecular biology, 432(7), 1978-1995 (2020-02-10)
Serum amyloid A (SAA) is a plasma protein that transports lipids during inflammation. To explore SAA solution conformations and lipid-binding mechanism, we used hydrogen-deuterium exchange mass spectrometry, lipoprotein reconstitution, amino acid sequence analysis, and molecular dynamics simulations. Solution conformations of
Ahmad Kiani Karanji et al.
Journal of mass spectrometry : JMS, 55(1), e4470-e4470 (2019-11-23)
The first 17 amino acid residues of Huntingtin protein (Nt17 of htt) are thought to play an important role in the protein's function; Nt17 is one of two membrane binding domains in htt. In this study the binding ability of
Yuko Fujioka et al.
Nature, 578(7794), 301-305 (2020-02-07)
Many biomolecules undergo liquid-liquid phase separation to form liquid-like condensates that mediate diverse cellular functions1,2. Autophagy is able to degrade such condensates using autophagosomes-double-membrane structures that are synthesized de novo at the pre-autophagosomal structure (PAS) in yeast3-5. Whereas Atg proteins
Luma da Costa Loureiro et al.
Molecular immunology, 122, 163-172 (2020-05-04)
Pulmonary surfactant plays an important role in lung surface tension, defense against invading pathogens, and immune response. Furthermore, alveolar macrophages (AM) that comprise the front line of immune defense against inhaled microorganisms are covered by a layer of pulmonary fluid.
Agnieszka Olżyńska et al.
Langmuir : the ACS journal of surfaces and colloids, 36(4), 1023-1033 (2020-01-07)
We introduce and study a multicomponent lipid film mimicking lipid composition of the human lung surfactant. It consists of phospholipids with various lipid headgroups and tail saturation. Furthermore, it includes cholesterol and oxidized lipids. Langmuir trough and fluorescence microscopy experiments
Celina Love et al.
Angewandte Chemie (International ed. in English) (2020-01-17)
In situ, reversible coacervate formation within lipid vesicles represents a key step in the development of responsive synthetic cellular models. Herein, we exploit the pH responsiveness of a polycation above and below its pKa , to drive liquid-liquid phase separation, to
Marta Martinez-Calle et al.
Biochimica et biophysica acta. Biomembranes, 1862(6), 183216-183216 (2020-02-19)
Surfactant protein SP-B is absolutely required for the generation of functional pulmonary surfactant, a unique network of multilayered membranes, which stabilizes the respiratory air-liquid interface. It has been proposed that SP-B assembles into hydrophobic rings and tubes that facilitate the
Julia Genova et al.
Life (Basel, Switzerland), 5(2), 1101-1110 (2015-03-31)
Thermally induced shape fluctuations were used to study elastic properties of giant vesicles composed of archaeal lipids C25,25-archetidyl (glucosyl) inositol and C25,25-archetidylinositol isolated from lyophilised Aeropyrum pernix K1 cells. Giant vesicles were created by electroformation in pure water environment. Stroboscopic
Wayne Mitchell et al.
The Journal of biological chemistry, 295(21), 7452-7469 (2020-04-11)
Mitochondrial dysfunction underlies many heritable diseases, acquired pathologies, and aging-related declines in health. Szeto-Schiller (SS) peptides comprise a class of amphipathic tetrapeptides that are efficacious toward a wide array of mitochondrial disorders and are believed to target mitochondrial membranes because
Eun-Young Lee et al.
Rejuvenation research, 18(3), 245-256 (2015-01-28)
Human growth hormone-1 (GH-1), somatotropin, is a peptide hormone that stimulates cell division in tissues such as bone and cartilage. To compare physiological activities in lipid-free and lipid-bound states, we expressed and incorporated GH-1 in reconstituted high-density lipoprotein (rHDL). GH-1
Sigalit Meker et al.
Langmuir : the ACS journal of surfaces and colloids, 34(32), 9548-9560 (2018-07-19)
Amyloid-β (Aβ) peptide has been implicated in Alzheimer's disease, which is a leading cause of death worldwide. The interaction of Aβ peptides with the lipid bilayers of neuronal cells is a critical step in disease pathogenesis. Recent evidence indicates that
Simli Dey et al.
Physical chemistry chemical physics : PCCP, 22(26), 14613-14620 (2020-06-03)
Oligomers are the key suspects in protein aggregation-linked diseases, such as Alzheimer's and Type II diabetes, and most likely exert their toxicity by interacting with lipid membranes. However, the "which oligomer" question remains an obstacle in understanding the disease mechanism
Renato M S Cardoso et al.
Biochimica et biophysica acta. Biomembranes, 1862(3), 183157-183157 (2019-12-18)
Association of amphiphiles with biomembranes is important for their availability at specific locations in organisms and cells, being critical for their biological function. A prominent role is usually attributed to the hydrophobic effect, and to electrostatic interactions between charged amphiphiles
Sophia C Goodchild et al.
PloS one, 9(8), e104492-e104492 (2014-08-08)
Although the molecular mechanisms underlying the pathology of amyloidoses are not well understood, the interaction between amyloid proteins and cell membranes is thought to play a role in several amyloid diseases. Amyloid fibrils of β2-microglobulin (β2m), associated with dialysis-related amyloidosis
Masato Abe et al.
Biochemistry, 56(47), 6268-6280 (2017-11-02)
Remodeling of the acyl chain compositions of cardiolipin (CL) species by the transacylase tafazzin is an important process for maintaining optimal mitochondrial functions. The results of mechanistic studies on the tafazzin-mediated transacylation from phosphatidylcholine (PC) to monolyso-CL (MLCL) in artificial
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