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Giuseppe Trapani et al.
Frontiers in bioengineering and biotechnology, 8, 427-427 (2020-05-28)
Engineered graphene-based derivatives are attractive and promising candidates for nanomedicine applications because of their versatility as 2D nanomaterials. However, the safe application of these materials needs to solve the still unanswered issue of graphene nanotoxicity. In this work, we investigated
Martin Gustavsson et al.
Methods in enzymology, 570, 233-260 (2016-02-29)
The development of methods for expression and purification of seven-transmembrane receptors has led to an increase in structural and biophysical data and greatly improved the understanding of receptor structure and function. For chemokine receptors, this has been highlighted by the
Luma da Costa Loureiro et al.
Molecular immunology, 122, 163-172 (2020-05-04)
Pulmonary surfactant plays an important role in lung surface tension, defense against invading pathogens, and immune response. Furthermore, alveolar macrophages (AM) that comprise the front line of immune defense against inhaled microorganisms are covered by a layer of pulmonary fluid.
Pramod Kumar et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(3), 1788-1798 (2020-01-09)
The lipid dependence of the nicotinic acetylcholine receptor from the Torpedo electric organ has long been recognized, and one of the most consistent experimental observations is that, when reconstituted in membranes formed by zwitterionic phospholipids alone, exposure to agonist fails
Guangtao Li et al.
Journal of lipid research, 61(5), 758-766 (2020-01-23)
The formation and properties of liquid-ordered (Lo) lipid domains (rafts) in the plasma membrane are still poorly understood. This limits our ability to manipulate ordered lipid domain-dependent biological functions. Giant plasma membrane vesicles (GPMVs) undergo large-scale phase separations into coexisting
Yuko Fujioka et al.
Nature, 578(7794), 301-305 (2020-02-07)
Many biomolecules undergo liquid-liquid phase separation to form liquid-like condensates that mediate diverse cellular functions1,2. Autophagy is able to degrade such condensates using autophagosomes-double-membrane structures that are synthesized de novo at the pre-autophagosomal structure (PAS) in yeast3-5. Whereas Atg proteins
Julia Genova et al.
Life (Basel, Switzerland), 5(2), 1101-1110 (2015-03-31)
Thermally induced shape fluctuations were used to study elastic properties of giant vesicles composed of archaeal lipids C25,25-archetidyl (glucosyl) inositol and C25,25-archetidylinositol isolated from lyophilised Aeropyrum pernix K1 cells. Giant vesicles were created by electroformation in pure water environment. Stroboscopic
John M Harrington et al.
The Journal of biological chemistry, 285(37), 28659-28666 (2010-07-10)
Trypanosoma brucei is the causative agent of both a veterinary wasting disease and human African trypanosomiasis, or sleeping sickness. The cell membrane of the developmental stage found within the mammalian host, the bloodstream form (BSF), is highly dynamic, exhibiting rapid
Xiaozheng Xu et al.
The Journal of cell biology, 219(6) (2020-05-22)
Blockade antibodies of the immunoinhibitory receptor PD-1 can stimulate the anti-tumor activity of T cells, but clinical benefit is limited to a fraction of patients. Evidence suggests that BTLA, a receptor structurally related to PD-1, may contribute to resistance to
Dominika Kozon et al.
Macromolecular bioscience, 19(12), e1900254-e1900254 (2019-11-21)
Polycations, mimicking activity of antibacterial peptides, belong to an important class of molecules investigated as a support or as an alternative to antibiotics. In this work, studies of modified linear amphiphilic statistical polymethyloxazoline (PMOX) and polyethyleneimine copolymers (PMOX_PEI) series are
Yo Yano et al.
Biophysical journal, 115(8), 1530-1540 (2018-10-03)
Sphingomyelin is an abundant lipid in some cellular membrane domains, such as lipid rafts. Hydrogen bonding and hydrophobic interactions of the lipid with surrounding components such as neighboring sphingomyelin and cholesterol (Cho) are widely considered to stabilize the raft-like liquid-ordered
Yasunori Saheki et al.
Nature cell biology, 18(5), 504-515 (2016-04-12)
Acute metabolic changes in plasma membrane (PM) lipids, such as those mediating signalling reactions, are rapidly compensated by homeostatic responses whose molecular basis is poorly understood. Here we show that the extended synaptotagmins (E-Syts), endoplasmic reticulum (ER) proteins that function
Charles G Cranfield et al.
Langmuir : the ACS journal of surfaces and colloids, 33(26), 6630-6637 (2017-06-14)
Cyclotides are cyclic disulfide-rich peptides that are chemically and thermally stable and possess pharmaceutical and insecticidal properties. The activities reported for cyclotides correlate with their ability to target phosphatidylethanolamine (PE)-phospholipids and disrupt cell membranes. However, the mechanism by which this
Sigalit Meker et al.
Langmuir : the ACS journal of surfaces and colloids, 34(32), 9548-9560 (2018-07-19)
Amyloid-β (Aβ) peptide has been implicated in Alzheimer's disease, which is a leading cause of death worldwide. The interaction of Aβ peptides with the lipid bilayers of neuronal cells is a critical step in disease pathogenesis. Recent evidence indicates that
Agnieszka Olżyńska et al.
Langmuir : the ACS journal of surfaces and colloids, 36(4), 1023-1033 (2020-01-07)
We introduce and study a multicomponent lipid film mimicking lipid composition of the human lung surfactant. It consists of phospholipids with various lipid headgroups and tail saturation. Furthermore, it includes cholesterol and oxidized lipids. Langmuir trough and fluorescence microscopy experiments
Sophia C Goodchild et al.
PloS one, 9(8), e104492-e104492 (2014-08-08)
Although the molecular mechanisms underlying the pathology of amyloidoses are not well understood, the interaction between amyloid proteins and cell membranes is thought to play a role in several amyloid diseases. Amyloid fibrils of β2-microglobulin (β2m), associated with dialysis-related amyloidosis
Renato M S Cardoso et al.
Biochimica et biophysica acta. Biomembranes, 1862(3), 183157-183157 (2019-12-18)
Association of amphiphiles with biomembranes is important for their availability at specific locations in organisms and cells, being critical for their biological function. A prominent role is usually attributed to the hydrophobic effect, and to electrostatic interactions between charged amphiphiles
Kelly E Burns et al.
Scientific reports, 6, 28465-28465 (2016-06-24)
Positively charged antimicrobial peptides have become promising agents for the treatment of cancer by inducing apoptosis though their preferential binding and disruption of negatively charged membranes, such as the mitochondrial membrane. (KLAKLAK)2 is such a peptide but due to its
Sarah N Sipe et al.
Analytical chemistry, 92(1), 899-907 (2019-11-26)
Development of chemical chaperones to solubilize membrane protein complexes in aqueous solutions has allowed for gas-phase analysis of their native-like assemblies, including rapid evaluation of stability and interacting partners. Characterization of protein primary sequence, however, has thus far been limited.
Sumie Manno et al.
The Journal of biological chemistry, 285(44), 33923-33929 (2010-08-21)
Human erythrocytes are continuously exposed to glucose, which reacts with the amino terminus of the β-chain of hemoglobin (Hb) to form glycated Hb, HbA1c, levels of which increase with the age of the circulating cell. In contrast to extensive insights
Priyanka Nair et al.
Archives of biochemistry and biophysics, 678, 108188-108188 (2019-11-13)
Non-enveloped viruses require membrane-penetrating peptides for gaining entry inside the cytoplasm of host cells during the early stages of infection. Although several such peptides have been identified as essential components for non-enveloped virus entry, the molecular mechanism of membrane destabilization
Tao Jiang et al.
Nature, 577(7789), 216-220 (2020-01-10)
Precise protein sequencing and folding are believed to generate the structure and chemical diversity of natural channels1,2, both of which are essential to synthetically achieve proton transport performance comparable to that seen in natural systems. Geometrically defined channels have been
Victoria M Latza et al.
Biophysical journal, 118(7), 1602-1611 (2020-02-26)
Membrane-bound oligosaccharides with specific chemistries are known to promote tight adhesion between adjacent membranes via the formation of weak saccharide bonds. However, in the literature, one can find scattered evidence that other, more abundant saccharide chemistries exhibit similar behavior. Here
Nicholas M Frame et al.
Journal of molecular biology, 432(7), 1978-1995 (2020-02-10)
Serum amyloid A (SAA) is a plasma protein that transports lipids during inflammation. To explore SAA solution conformations and lipid-binding mechanism, we used hydrogen-deuterium exchange mass spectrometry, lipoprotein reconstitution, amino acid sequence analysis, and molecular dynamics simulations. Solution conformations of
Ahmad Kiani Karanji et al.
Journal of mass spectrometry : JMS, 55(1), e4470-e4470 (2019-11-23)
The first 17 amino acid residues of Huntingtin protein (Nt17 of htt) are thought to play an important role in the protein's function; Nt17 is one of two membrane binding domains in htt. In this study the binding ability of
Max Piffoux et al.
ACS nano, 12(7), 6830-6842 (2018-07-06)
Extracellular vesicles (EVs) are recognized as nature's own carriers to transport macromolecules throughout the body. Hijacking this endogenous communication system represents an attractive strategy for advanced drug delivery. However, efficient and reproducible loading of EVs with therapeutic or imaging agents
Y K Shin et al.
Biophysical journal, 61(6), 1443-1453 (1992-06-01)
A new general method for the determination of electrostatic potentials at biological surfaces is presented. The approach is based on measurement of the collision frequency of a charged nitroxide in solution with a nitroxide fixed to the surface at the
Lucile Guyot et al.
Current protocols in protein science, 100(1), e104-e104 (2020-04-15)
Pichia pastoris is a eukaryotic microorganism reputed for its ability to mass-produce recombinant proteins, including integral membrane proteins, for various applications. This article details a series of protocols that progress towards the production of integral membrane proteins, their extraction and
David A Bryce et al.
Analytical chemistry, 90(19), 11509-11516 (2018-09-04)
Interactions of lectins, proteins that selectively bind carbohydrates, play an important role in many biological processes including cell adhesion, immune response, and cell signaling. Given the range of lectin functions and their potential for application in disease detection, there is
Karolina Piecyk et al.
Bioconjugate chemistry, 31(4), 1156-1166 (2020-04-02)
The development of targeted anticancer drugs has been one of the most challenging goals of current research. Eukaryotic translation initiation factor 4E (eIF4E) is an oncogene that stimulates mRNA translation via binding to the 5' endcap structure. It is well
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