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Lingzhi Liu et al.
Biosensors & bioelectronics, 70, 351-357 (2015-04-05)
Measurement of microRNA (miRNA) levels in body fluids is a crucial tool for the early diagnosis and prognosis of cancers. In this study, we developed an electrochemical assay to detect miRNA-21 by fabricating the electrode with layer-by-layer assembly of oxidized
Cynthia M Chio et al.
Chembiochem : a European journal of chemical biology, 16(12), 1735-1739 (2015-06-13)
Few chemical strategies for activating enzymes have been developed. Here we show that a biarsenical compound (FlAsH) can directly activate a rationally engineered protein tyrosine phosphatase (Shp2 PTP) by disrupting autoinhibitory interactions between Shp2's N-terminal SH2 domain and its PTP
Yan Li et al.
Biosensors & bioelectronics, 67, 364-369 (2014-09-10)
The development of sensitive telomerase biosensors is hindered by the restricted accessibility of telomere strand (TS) primer and the limited enzyme reaction space, which is mainly confined by the vertical distance. In this work, we designed an electrochemical telomerase biosensor
Muwen Kong et al.
Molecular cell, 79(1), 99-114 (2020-05-24)
Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human
Benjamin D Stein et al.
Cell reports, 29(10), 3331-3348 (2019-12-05)
Metformin is the front-line treatment for type 2 diabetes worldwide. It acts via effects on glucose and lipid metabolism in metabolic tissues, leading to enhanced insulin sensitivity. Despite significant effort, the molecular basis for metformin response remains poorly understood, with
Dietmar Reusch et al.
mAbs, 7(4), 732-742 (2015-05-23)
To monitor the Fc glycosylation of therapeutic immunoglobulin G in bioprocess development, product characterization and release analytics, reliable techniques for glycosylation analysis are needed. Several analytical methods are suitable for this application. We recently presented results comparing detection methods for
Zsuzsanna Darula et al.
Analytical chemistry, 87(12), 6297-6302 (2015-05-16)
Iodoacetamide is perhaps the most widely used reagent for the alkylation of free sulfhydryls in proteomic experiments. Here, we report that both incomplete derivatization of Cys side chains and overalkylation of the peptides may lead to the misassignment of glycoforms
Matthew S P Boyles et al.
Journal of nanobiotechnology, 13, 84-84 (2015-11-20)
Gold nanoparticles (AuNPs) are a popular choice for use in medical and biomedical research applications. With suitable functionalisation AuNPs can be applied in drug delivery systems, or can aid in disease diagnosis. One such functionalisation is with chitosan, which enables
Grigory Ryzhakov et al.
Nature communications, 12(1), 6702-6702 (2021-11-20)
Interferon regulating factor 5 (IRF5) is a multifunctional regulator of immune responses, and has a key pathogenic function in gut inflammation, but how IRF5 is modulated is still unclear. Having performed a kinase inhibitor library screening in macrophages, here we
Chiharu Mizuguchi et al.
Biochimica et biophysica acta, 1841(12), 1716-1724 (2014-10-05)
Human apolipoprotein E (apoE) isoforms exhibit different conformational stabilities and lipid-binding properties that give rise to altered cholesterol metabolism among the isoforms. Using Trp-substituted mutations and site- directed fluorescence labeling, we made a comprehensive comparison of the conformational organization of
Sean Chen et al.
Cell reports, 27(13), 3780-3789 (2019-06-27)
Genetically engineered mouse models harboring large sequence insertions or modifications are critical for a wide range of applications including endogenous gene tagging, conditional knockout, site-specific transgene insertion, and gene replacement; however, existing methods to generate such animals remain laborious and
Multiomics Reveals Ectopic ATP Synthase Blockade Induces Cancer Cell Death via a lncRNA-mediated Phospho-signaling Network.
Chang, et al.
Molecular and Cellular Proteomics, 19, 1805-1825 (2021)
Zaur M Kachaev et al.
FEBS letters, 593(10), 1102-1112 (2019-04-20)
The Paip2 protein is a factor regulating mRNA translation and stability in the cytoplasm. It has also been found in the nuclei of several cell types in Drosophila. Here, we aim to elucidate the functions of Paip2 in the cell
Rui Sun et al.
Journal of proteome research, 19(7), 2732-2741 (2020-02-14)
We reported and evaluated a microflow, single-shot, short gradient SWATH MS method intended to accelerate the discovery and verification of protein biomarkers in preclassified clinical specimens. The method uses a 15 min gradient microflow-LC peptide separation, an optimized SWATH MS
Torsten Müller et al.
Molecular systems biology, 16(1), e9111-e9111 (2020-03-05)
High-throughput and streamlined workflows are essential in clinical proteomics for standardized processing of samples from a variety of sources, including fresh-frozen tissue, FFPE tissue, or blood. To reach this goal, we have implemented single-pot solid-phase-enhanced sample preparation (SP3) on a
Punam Sonar et al.
Current biology : CB, 30(6), 1160-1166 (2020-03-07)
Construction and function of virtually all cilia require the universally conserved process of intraflagellar transport (IFT) [1, 2]. During the atypically fast IFT in the green alga C. reinhardtii, on average, 10 kinesin-2 motors "line up" in a tight assembly on
Yu Wang et al.
Bio-protocol, 10(18), e3767-e3767 (2021-03-05)
B lymphocyte activation is regulated by its membrane-bound B cell receptors (BCRs) upon recognizing diverse antigens. It is hypothesized that antigen binding would trigger conformational changes within BCRs, followed by a series of downstream signaling activation. To measure the BCR
Meriem Ameziani et al.
The protein journal, 39(5), 574-590 (2020-09-23)
The current report focuses on purification, structural and functional characterization of Cerastategrin from Cerastes cerastes venom, a novel basic disintegrin (pI 8.36) with 128 amino acid residues and a molecular weight of 13 835.25 Da measured by MALDI-MSMS. The 3D structure
Qi Li et al.
Cell stem cell, 26(5), 675-692 (2020-04-08)
Intestinal homeostasis is tightly regulated by complex yet poorly understood signaling networks. Here, we demonstrate that Lats1/2, the core Hippo kinases, are essential to maintain Wnt pathway activity and intestinal stem cells. Lats1/2 deletion leads to loss of intestinal stem
Felista Tansi et al.
Journal of cellular biochemistry, 116(7), 1222-1231 (2014-12-30)
The internalization of near-infrared fluorescently labeled cargos into living cells and tissues allows a highly sensitive detection without interference from skin, porphins or other fluorescent cell and tissue compounds. In this study, the uptake of labeled bovine serum albumin and
Remi Hatinguais et al.
Frontiers in immunology, 12, 641495-641495 (2021-04-13)
Reactive Oxygen Species (ROS) are highly reactive molecules that can induce oxidative stress. For instance, the oxidative burst of immune cells is well known for its ability to inhibit the growth of invading pathogens. However, ROS also mediate redox signalling
Yvonne S Ziegler et al.
PloS one, 9(7), e102341-e102341 (2014-07-17)
The use of broad spectrum chemotherapeutic agents to treat breast cancer results in substantial and debilitating side effects, necessitating the development of targeted therapies to limit tumor proliferation and prevent metastasis. In recent years, the list of approved targeted therapies
Suman Rao et al.
Cell chemical biology, 26(6), 818-829 (2019-04-16)
Covalent kinase inhibitors, which typically target cysteine residues, represent an important class of clinically relevant compounds. Approximately 215 kinases are known to have potentially targetable cysteines distributed across 18 spatially distinct locations proximal to the ATP-binding pocket. However, only 40
Tris (2-carboxyethyl) phosphine Hydrochloride
Yost Julianne M et al.
Encyclopedia of Reagents for Organic Synthesis, Second Edition, 1-9 (2001)
Barbora Salovska et al.
Molecular systems biology, 16(3), e9170-e9170 (2020-03-17)
Profiling of biological relationships between different molecular layers dissects regulatory mechanisms that ultimately determine cellular function. To thoroughly assess the role of protein post-translational turnover, we devised a strategy combining pulse stable isotope-labeled amino acids in cells (pSILAC), data-independent acquisition
Maria Chiara Barsotti et al.
Journal of biomedical materials research. Part A, 103(10), 3284-3292 (2015-03-27)
As the endothelium still represents the ideal surface for cardiovascular devices, different endothelialization strategies have been attempted for biocompatibility and nonthrombogenicity enhancement. Since endothelial progenitor cells (EPCs) could accelerate endothelialization, preventing thrombosis and restenosis, the aim of this study was
Huiyan Li et al.
Talanta, 146, 727-731 (2015-12-24)
We present an aptamer-based microcantilever biosensor for label-free detection of nucleolin. The sensor cantilevers in the microcantilever array were functionalized with nucleolin aptamer (AS1411) while the reference cantilevers were modified by 6-mercapto-1-hexanol (MCH) to eliminate environmental disturbances. The interaction between
Patanachai Limpikirati et al.
mAbs, 11(3), 463-476 (2019-01-15)
Monoclonal antibodies are among the fastest growing therapeutics in the pharmaceutical industry. Detecting higher-order structure changes of antibodies upon storage or mishandling, however, is a challenging problem. In this study, we describe the use of diethylpyrocarbonate (DEPC)-based covalent labeling (CL)
Hui Chan Kwak et al.
Biochemical pharmacology, 96(3), 256-266 (2015-06-07)
The present study was aimed to investigate the metabolomics of sulfur amino acids in Zucker diabetic fatty (ZDF) rats, an obese type 2 diabetic animal model. Plasma levels of total cysteine, homocysteine and methionine, but not glutathione (GSH) were markedly
Karl T Hansson et al.
Proteomics, 17(5) (2017-01-04)
Biomarkers of neurodegenerative disorders are needed to assist in diagnosis, to monitor disease progression and therapeutic interventions, and to provide insight into disease mechanisms. One route to identify such biomarkers is by proteomic and peptidomic analysis of cerebrospinal fluid (CSF).
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