ELISA

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Technical support for Secondary Antibody: How to Choose a Secondary Antibody.

Troubleshoot and optimize ELISAs using this guide that includes solutions to some of the most common sources of problems for assay development.

Antibody Generation

The inherent immune ability of an animal can be leveraged to generate antibodies that bind to specific molecules.

Secondary Antibodies, Conjugates, and Kits

Technical Support for Secondary Antibodies, Conjugates, and Kits

Antibody Standard Validation

Explore antibody validation, including antibody and target, species validation, lot validation, application validation, storage and handling, citations, and quality control, in detail.

Conferma^® ELISAs: Designed for Sample Detection & Lot Consistency

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

Prestige Antibodies® in Breast Cancer Research

The Human Protein Atlas; Prestige Antibodies®; Polyclonals and Monoclonals; Clinical Markers; Antibodies used in Breast Cancer Research; Antibodies Against MammaPrint and Other Gene Expression Test Proteins; Antibodies Identified in the Human Protein Atlas; Finding Cancer Biomarkers, as Exemplified by RBM3

Antibody Enhanced-Validation Strategies

Antibody enhanced validation provides additional data to help ensure antibody specificity and performance and to address antibody reproducibility crisis.

An Introduction to Antibodies: Antibody-Antigen Interaction

The strength of interaction between antibody and antigen at single antigenic sites can be described by the affinity of the antibody for the antigen.

A Highly Sensitive IFN-γ ELISpot Assay to Quantify Cellular Immune Responses to Previous Viral Infection

This study presents an example of a typical ELISpot assay for quantifying the number of pre-existing, antigen-reactive T cells from peripheral blood mononuclear cell (PBMC) samples obtained from donors previously infected with cytomegalovirus (CMV).

Antibodies in Practice: Conjugated and Secondary Antibody Use

Using conjugated antibodies and secondary antibodies in the lab.

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

Protein Conformation Array Multiplexing

Monoclonal antibodies (mAbs) are the fastest growing class of human therapeutics within the field of biologics, having predicted worldwide sales of $125 billion by 2020 based on current approval rates.

An Introduction to Antibodies: Antigens, Epitopes and Antibodies

Antibodies will combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond, and the use of this robust and specific binding as a molecular tag for research.

The ELISpot Assay Enables Functional Analysis of Cellular Immunology

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

Prestige Antibodies® Organelle Markers

Subcellular localization studies are important for mapping and characterizing proteins and thus for better understanding of the cellular functions of the proteins. By confocal microscopy analysis on human cell lines, spatial and temporal protein expression patterns can be visualized on

Complete Solutions for IVD Chemiluminescent Immunoassay (CLIA/CLEIA) Development

Accelerate your time to market with fit-for-use products which offer the quality, consistency, and documentation necessary for every step of your IVD development and manufacturing needs.

Antibody Basics

Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.