Merck
Search Within
Applications
Content Type
Applied Filters:
Applications:Gene expression and silencing
Content Type:Technical Article
Showing 1-30 of 76 results
Sort by Relevance
Molecular Beacons
Molecular Beacons are structured probes that are highly sensitive, sequence specific, and are used for sequence detection in qPCR and in vitro studies.
siRNA Delivery in Mammalian Cell Lines
siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.
MISSION® Lentiviral shRNA
MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.
Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library
The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.
Using Probe-Based Quantitative PCR (Qpcr) to Measure Gene-Level Expression
Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer
Safe Lentivirus Handling
Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.
Phosphorothioate Oligonucleotides
Phosphorothioate is a widely used backbone variant effective in creating oligonucleotides for antisense knockdowns.
General Adherent Cells
Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.
Reverse Transcription
One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and
Antisense Oligonucleotides
This article summarizes several of the common mechanisms of antisense gene modulation and more importantly, considerations to take into account when designing an antisense oligonucleotide (ASO).
Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
DNA Oligonucleotide Synthesis
Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis.
MISSION® shRNA Library: Next Generation RNAi
RNAi technology has been one of the key biological breakthroughs of the last decade and has revolutionized basic biology, gene function studies, and it also holds promise to revolutionize drug discovery and therapeutics. Synthetic siRNAs have delivered the genetic
MISSION® FAQs
We answer your frequently asked questions about shRNA including available formats, experimental design and analysis, advanced applications, ordering, custom options and much more.
MISSION® Target ID Library for Human miRNA Target ID and Discovery
MISSION® Target ID Library for Human miRNA Target Identification and Discovery;
Lentivirus Cell Line
Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.
Custom Services
We are the exclusive distributor of the TRC shRNA library clones in DNA or lentiviral format and many custom services are available.
Lenti GoClone
Validate miRNA targets and study post-transcriptional gene regulation with lentiviral 3'UTR reporters.
MISSION® shRNA Lentiviral Vectors Summary
RNA interference technology has been one of the key biological breakthroughs of the last decade and has revolutionized basic biology and gene function studies, but it also holds promise to revolutionize drug discovery and therapeutics.
Predesigned siRNA
We offer advanced design and quality synthesis for predesigned siRNA and siRNA libraries using the Rosetta siRNA Design Algorithm to reduce off-target effects and increase RNAi performance. The algorithm utilizes Position-Specific Scoring Matrices (PSSM) and knowledge of the siRNA seed
X-tremeGENE™ siRNA Transfection Reagent: A Powerful Tool for Antisense Inhibition of miRNA in Human Cells with miRCURY Knockdown Probes
MicroRNAs (miRNAs) are an endogenous class of RNA molecules comprising 19-25 nucleotides that regulate the suitability or transflational efficiency of mRNAs.
esiRNA FAQs (Frequently Asked Questions)
Custom & Predesigned esiRNA FAQ
MISSION® siRNA FAQs
We answer your frequently asked questions about siRNA including experimental design and analysis, advanced applications, custom options and much more.
Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells
In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.
shRNA FAQs
We answers your frequently asked questions about shRNA, the TRC. MISSION and more.
Efficient Gene Silencing using MISSION® TRC shRNA Lentiviral Vectors
The RNAi Consortium (TRC) is a collaborative effort among several academic laboratories (including the Broad Institute, the Whitehead Institute for Biomedical Research, the Dana Farber Cancer Institute, Massachusetts General Hospital, Massachusetts Institute of Technology, Harvard Medical School, Columbia University, and
Utility of shRNA Clones
A complimentary approach to overexpression experiments is to observe the effects of F-Box Protein 6 (i.e. FBG2 or FBXO6) specific suppression on A1AT levels. A panel of FBG2 specific shRNA plasmids was transfected into COS-7 cells in equimolar amounts with
Lentiviral Transduction
Methods for Enhancing Lentiviral Transduction Efficiency
Retinoic Acid and Gene Expression
All-trans retinoic acid (RA, ATRA) is a pleiotropic activation factor that regulates genes associated with normal vertebrate cellular processes such as cell differentiation, cell proliferation, apoptosis, and embryonic development.
MISSION® Application Data
MISSION® Application Data. We are committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries.
Page 1 of 3
Page 1 of 3