Mammalian cell culture

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Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.

BRAND® Insert 2in1 Supports the Cultivation of Reconstructed Human Epidermis (RhE) used for Skin Corrosion Tests (OECD TG 431)

The BRAND® Insert 2in1 cell culture insert supports the cultivation of Reconstructed Human Epidermis used for skin corrosion tests.

3D Neurosphere Culture Media

3D neurosphere culture protocol to expand human U-87 MG glioblastoma cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

Iscove's Modified Dulbecco's Media (IMDM) Formulation

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

McCoy's 5A Modified Media Formulation

Copper in Cell Culture

Importance and uses of copper in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO), cell cultures

Hepatocyte Growth Factor (HGF)

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.

Cell Death Troubleshooting in Cell Culture

Here we discuss causes of cell death, and how to assess and prevent these causes.

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

Linoleic acid in Cell Culture

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Linolenic Acid in Cell Culture

Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Citrate Serum-Free Medium Supplement

Citrate is one of many cell culture components that affects the performance of serum-free, protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins such as monoclonal antibodies.

Mammary Epithelial Cell Growth Medium

Serum-free Mammary Epithelial Cell Growth Media optimized for the in vitro cultivation of epithelial cells from the mammary gland. Protocols for handling, cell passaging, media, and product use.

Alternative High-Yield Cell Culture Systems

Solutions for high-yield cell culture scale-up applications including multi-layered flasks (HyperFlasks/CellStacks), roller bottles, spinner and shaker flasks. Free ECACC handbook download.

Oleic Acid in Cell Culture

How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

Media Formulations

Compare all of the formulation tables for cell culture in one place.

Dulbecco's Modified Eagle's Medium (DMEM) Formulation

L-Tyrosine in Cell Culture

Learn more about the amino acid L-Tyrosine and explore how it is used in cell culture media like MEM, DMEM, and RPMI. We offer a comprehensive portfolio of amino acid cell culture supplements and reagents.

β-Alanine in Cell Culture

Learn more about β-Alanine, an important amino acid supplement and explore how it is used in cell culture. We offer a comprehensive portfolio of amino acid cell culture supplements and reagents.

DMEM/nutrient Mixture F-12 Formulation

Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F-12 (DME/F12) Formulation

Cell Line Cross-Contamination and Misidentification; Common Cell Culture Problems

Cell line cross-contamination and misidentification can be solved with authentication and conscientious sourcing.

Glucose in Cell Culture

Importance and uses of glucose in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Ferric and Ferrous Iron in Cell Culture

Importance and uses of ferric and ferrous iron in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Using Immortalized 16HBE14o- Human Bronchial Epithelial Cell Lines to Model Respiratory Lung Diseases

16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.

Cell Culture Fundamentals: Safety Aspects of Cell Culture

Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

Corning® High Content Screening Microplates – Frequently Asked Questions

A frequently asked questions guide for users of the Corning® high content screening microplates.

Epidermal Growth Factor Receptor (EGFR) Signaling

Review EGF/EGFR signaling (epidermal growth factor receptor) and find links to related products.

Vitamin B12, Cobalamin, in Cell Culture

Importance and uses of Vitamin B12 in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell cultures

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