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Troubleshooting Strategies in GST-tagged Protein Purification
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Sera-Mag™ and Sera-Mag SpeedBeads Magnetic Particles
Sera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays.
Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity
A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.
Agarose Beads Vs. Magnetic Beads in ChIP
Agarose beads Vs. Magnetic beads in Chromatin Immunoprecipitation (ChIP)
Centrifugation Separations
CsCl gradient centrifugation separates RNA from DNA; differential and density gradient centrifugation techniques explained.
Protein G and Protein A Bind to Different IgG
This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.
Robust, load independent viral clearance in monoclonal antibody purification with Natrix® Q chromatography membrane
Membrane adsorbers are a cost-effective, robust, single-use solution for monoclonal antibody polishing
Immunoprecipitation Techniques
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
Column Packing and Preparation for Affinity Chromatography of Antibodies
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
Centrifugation Basics
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.