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Proteins on Supported Lipid Bilayers Diffusing around Proteins Fixed on Acrylate Anchors.

Analytical chemistry (2018-10-24)
Bianca Buchegger, Johannes Kreutzer, Markus Axmann, Sandra Mayr, Richard Wollhofen, Birgit Plochberger, Jaroslaw Jacak, Thomas A Klar
ABSTRACT

Mobility of proteins and lipids plays a major role in physiological processes. Platforms which were developed to study protein interaction between immobilized and mobile proteins suffer from shortcomings such as fluorescence quenching or complicated fabrication methods. Here we report a versatile platform comprising immobilized histidine-tagged proteins and biotinylated proteins in a mobile phase. Importantly, multiphoton photolithography was used for easy and fast fabrication of the platform and allows, in principle, extension of its application to three dimensions. The platform, which is made up of functionalized polymer structures embedded in a mobile lipid bilayer, shows low background fluorescence and allows for mobility of arbitrary proteins.

MATERIALS
Product Number
Brand
Product Description

Avanti
16:0-18:1 PC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, chloroform
Avanti
16:0-18:1 PC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, powder
Avanti
16:0 Biotinyl Cap PE, Avanti Polar Lipids 870277P, powder