Merck

Perfluorinated compounds affect the function of sex hormone receptors.

Environmental science and pollution research international (2013-06-15)
Lisbeth Stigaard Kjeldsen, Eva Cecilie Bonefeld-Jørgensen
ABSTRACT

Perfluorinated compounds (PFCs) are a large group of chemicals used in different industrial and commercial applications. Studies have suggested the potential of some PFCs to disrupt endocrine homeostasis, increasing the risk of adverse health effects. This study aimed to elucidate mechanisms behind PFC interference with steroid hormone receptor functions. Seven PFCs [perfluorohexane sulfonate (PFHxS), perfluorooctane sulfonate (PFOS), perfluorooctanoate (PFOA), perfluorononanoate (PFNA), perfluorodecanoate (PFDA), perfluoroundecanoate (PFUnA), and perfluorododecanoate (PFDoA)] were analyzed in vitro for their potential to affect estrogen receptor (ER) and androgen receptor (AR) transactivity as well as aromatase enzyme activity. The PFCs were assessed as single compounds and in an equimolar mixture. PFHxS, PFOS and PFOA significantly induced the ER transactivity, whereas PFHxS, PFOS, PFOA, PFNA and PFDA significantly antagonized the AR activity in a concentration-dependent manner. Moreover, PFDA weakly decreased the aromatase activity at a high test concentration. A mixture effect more than additive was observed on AR function. We conclude that five of the seven PFCs possess the potential in vitro to interfere with the function of the ER and/or the AR. The observed mixture effect emphasizes the importance of considering the combined action of PFCs in future studies to assess related health risks.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pentadecafluorooctanoic acid ammonium salt, ≥98.0% (NT)
Sigma-Aldrich
Heptadecafluorooctanesulfonic acid solution, ~40% in H2O (T)
Supelco
Heptadecafluorooctanesulfonic acid solution, 100 μg/mL in methanol, analytical standard
Sigma-Aldrich
Perfluorooctanoic acid, 95%
Sigma-Aldrich
Tetradecafluorohexane, A mixture of perfluorinated hexanes, 95%
Sigma-Aldrich
Tetradecafluorohexane, 99%
Supelco
Pentadecafluorooctanoic acid, analytical standard
Supelco
Pentadecafluorooctanoic acid solution, 100 μg/mL in methanol, analytical standard