Merck

Accumulation of perfluoroalkyl substances in human tissues.

Environment international (2013-07-31)
Francisca Pérez, Martí Nadal, Alícia Navarro-Ortega, Francesc Fàbrega, José L Domingo, Damià Barceló, Marinella Farré
ABSTRACT

Perfluoroalkyl substances (PFASs) are environmental pollutants with an important bioaccumulation potential. However, their metabolism and distribution in humans are not well studied. In this study, the concentrations of 21 PFASs were analyzed in 99 samples of autopsy tissues (brain, liver, lung, bone, and kidney) from subjects who had been living in Tarragona (Catalonia, Spain). The samples were analyzed by solvent extraction and online purification by turbulent flow and liquid chromatography coupled to tandem mass spectrometry. The occurrence of PFASs was confirmed in all human tissues. Although PFASs accumulation followed particular trends depending on the specific tissue, some similarities were found. In kidney and lung, perfluorobutanoic acid was the most frequent compound, and at highest concentrations (median values: 263 and 807ng/g in kidney and lung, respectively). In liver and brain, perfluorohexanoic acid showed the maximum levels (median: 68.3 and 141ng/g, respectively), while perfluorooctanoic acid was the most contributively in bone (median: 20.9ng/g). Lung tissues accumulated the highest concentration of PFASs. However, perfluorooctane sulfonic acid and perfluorooctanoic acid were more prevalent in liver and bone, respectively. To the best of our knowledge, the accumulation of different PFASs in samples of various human tissues from the same subjects is here reported for the very first time. The current results may be of high importance for the validation of physiologically based pharmacokinetic models, which are being developed for humans. However, further studies on the distribution of the same compounds in the human body are still required.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Heptadecafluorooctanesulfonic acid solution, ~40% in H2O (T)
Sigma-Aldrich
Pentadecafluorooctanoic acid ammonium salt, ≥98.0% (NT)
Sigma-Aldrich
Perfluorooctanoic acid, 95%
Supelco
Heptadecafluorooctanesulfonic acid solution, 100 μg/mL in methanol, analytical standard
Supelco
Heptafluorobutyric acid solution, 0.5 M in H2O, LiChropur, suitable for ion pair chromatography
Supelco
Perfluorobutanoic acid, analytical standard
Sigma-Aldrich
Heptafluorobutyric acid, 98%
Supelco
Heptafluorobutyric acid, suitable for ion chromatography, ≥99.5% (GC)
Sigma-Aldrich
Heptafluorobutyric acid, ≥99.0% (GC)
Supelco
Pentadecafluorooctanoic acid, analytical standard
Supelco
Pentadecafluorooctanoic acid solution, 100 μg/mL in methanol, analytical standard