- Polymer enzyme conjugates as chiral ligands for sharpless dihydroxylation of alkenes in organic solvents.
Polymer enzyme conjugates as chiral ligands for sharpless dihydroxylation of alkenes in organic solvents.
Chembiochem : a European journal of chemical biology (2014-11-26)
Stefan Konieczny, Melanie Leurs, Joerg C Tiller
PMID25421303
ABSTRACT
Conjugates of enzymes and poly(2-methyloxazoline) were used as organosoluble amphiphilic polymer nanocontainers for dissolving osmate, thereby converting the enzymes into organosoluble artificial metalloenzymes. These were shown to catalyze the dihydroxylation of different alkenes with high enantioselectivity. The highest selectivities, found for osmate complexed with laccase polymer-enzyme conjugates (PECs), even exceed those of classical Sharpless catalysts.
MATERIALS
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Brand
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Chloroform, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
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Chloroform, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 0.5-1.0% ethanol as stabilizer
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Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
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Diethyl ether, ACS reagent, ≥98.0%, contains ≤2% ethanol and ≤10ppm BHT as inhibitor