Merck
  • Home
  • Search Results
  • Metabolic changes in paraquat poisoned patients and support vector machine model of discrimination.

Metabolic changes in paraquat poisoned patients and support vector machine model of discrimination.

Biological & pharmaceutical bulletin (2015-03-12)
Xianqin Wang, Meiling Zhang, Jianshe Ma, Yuan Zhang, Guangliang Hong, Fa Sun, Guanyang Lin, Lufeng Hu
ABSTRACT

Numerous people die of paraquat (PQ) poisoning every year in the world. Although several studies regarding paraquat (PQ) poisoning have been conducted, the metabolic changes in plasma remain unknown. In this study, the metabolomics of 15 PQ poisoned patients with plasma PQ concentrations in excess of 0.1 µg/mL and 16 healthy volunteers were investigated. The plasma samples were evaluated through the use of gas chromatography-mass spectrometry (GC/MS) and analyzed by partial least-squares discriminant analysis (PLS-DA). Based on the metabolomics data, a support vector machine (SVM) discrimination model was developed. The results showed the plasma levels of urea, glucose oxime and L-phenylalanine decreased and cholesterol increased in PQ poisoned patients in comparison to healthy volunteers. The SVM discrimination model was developed, and performed with a high degree of accuracy, to distinguish PQ poisoned patients from healthy volunteers. In conclusion, metabolic pathways including the urea cycle, and amino acid, glucose, and cholesterol metabolism were impaired after PQ poisoning. An SVM discrimination model, based on metabolomics data, was established and may become a new powerful tool for the diagnosis of PQ poisoning.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Heptane, anhydrous, 99%
Supelco
N-Methyl-bis(trifluoroacetamide), for GC derivatization, LiChropur, ≥97.0% (GC)
Sigma-Aldrich
Chlorotrimethylsilane, purified by redistillation, ≥99%
Sigma-Aldrich
Chlorotrimethylsilane, ≥98.0% (GC)
Sigma-Aldrich
Chlorotrimethylsilane, puriss., ≥99.0% (GC)
Sigma-Aldrich
Chlorotrimethylsilane, produced by Wacker Chemie AG, Burghausen, Germany, ≥99.0% (GC)
Sigma-Aldrich
Heptane, biotech. grade, ≥99%
Supelco
Heptane, analytical standard
Supelco
N-Methyl-bis(trifluoroacetamide), for GC derivatization, LiChropur, ≥99.0% (GC)
Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (v/v) trifluoroacetic acid
Supelco
Chlorotrimethylsilane, for GC derivatization, LiChropur, ≥99.0% (GC)
Sigma-Aldrich
Heptane, HPLC Plus, for HPLC, GC, and residue analysis, 99%
Sigma-Aldrich
Heptane, suitable for HPLC, ≥96%
Sigma-Aldrich
Heptane, suitable for HPLC, ≥99%
Sigma-Aldrich
Heptane, ReagentPlus®, 99%
Supelco
Residual Solvent - Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Heptane, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
N-Methylhydroxylamine hydrochloride, 98%
Sigma-Aldrich
Chlorotrimethylsilane solution, 1.0 M in THF
Sigma-Aldrich
Acetic anhydride, ACS reagent, ≥98.0%
Sigma-Aldrich
Pyridine, ≥99%
Sigma-Aldrich
Pyridine, anhydrous, 99.8%
Sigma-Aldrich
Acetic anhydride, 99.5%
Sigma-Aldrich
Acetic anhydride, ≥99%
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
Sigma-Aldrich
N-Methyltrifluoroacetamide, 98%
Supelco
Acetonitrile, analytical standard
Supelco
Pyridine, analytical standard
Supelco
Acetic anhydride, for GC derivatization, LiChropur, ≥99.0%
Sigma-Aldrich
Acetonitrile, ACS reagent, ≥99.5%