Merck
  • Home
  • Search Results
  • Change in N-Glycosylation of Plasma Proteins in Japanese Semisupercentenarians.

Change in N-Glycosylation of Plasma Proteins in Japanese Semisupercentenarians.

PloS one (2015-11-13)
Yuri Miura, Noritaka Hashii, Hiroki Tsumoto, Daisuke Takakura, Yuki Ohta, Yukiko Abe, Yasumichi Arai, Nana Kawasaki, Nobuyoshi Hirose, Tamao Endo
ABSTRACT

An N-glycomic analysis of plasma proteins was performed in Japanese semisupercentenarians (SSCs) (mean 106.7 years), aged controls (mean 71.6 years), and young controls (mean 30.2 years) by liquid chromatography/mass spectrometry (LC/MS) using a graphitized carbon column. Characteristic N-glycans in SSCs were discriminated using a multivariate analysis; orthogonal projections to latent structures (O-PLS). The results obtained showed that multi-branched and highly sialylated N-glycans as well as agalacto- and/or bisecting N-glycans were increased in SSCs, while biantennary N-glycans were decreased. Since multi-branched and highly sialylated N-glycans have been implicated in anti-inflammatory activities, these changes may play a role in the enhanced chronic inflammation observed in SSCs. The levels of inflammatory proteins, such as CRP, adiponectin, IL-6, and TNF-α, were elevated in SSCs. These results suggested that responses to inflammation may play an important role in extreme longevity and healthy aging in humans. This is the first study to show that the N-glycans of plasma proteins were associated with extreme longevity and healthy aging in humans.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Guanidine hydrochloride solution
Sigma-Aldrich
Guanidine hydrochloride solution, BioUltra, ~8 M in H2O
Sigma-Aldrich
DL-Dithiothreitol solution, BioUltra, for molecular biology, ~1 M in H2O
Sigma-Aldrich
Carbon, mesoporous, average pore diameter 100±10 Å (typical), >99.95% trace metals basis
Sigma-Aldrich
Carbon, mesoporous, nanopowder, <500 nm particle size (DLS), >99.95% trace metals basis
Sigma-Aldrich
Carbon, mesoporous, nanopowder, graphitized, <500 nm particle size (DLS), >99.95% trace metals basis
Sigma-Aldrich
N-Acetylneuraminic acid, synthetic, ≥95%
Sigma-Aldrich
N-Acetylneuraminic acid, from Escherichia coli, ≥98%
Supelco
DL-Dithiothreitol solution, 1 M in H2O
Sigma-Aldrich
Carbon, mesoporous, hydrophilic pore surface
Sigma-Aldrich
Carbon, mesoporous
Sigma-Aldrich
Activated Charcoal Norit®, Norit® PK 1-3, from peat, steam activated, granular
Sigma-Aldrich
Activated Charcoal Norit®, Norit® RB3, for gas purification, steam activated, rod
Sigma-Aldrich
Activated Charcoal Norit®, Norit® SX2, powder, from peat, multi-purpose activated charcoal, steam activated and acid washed
Sigma-Aldrich
Activated Charcoal Norit®, Norit® CA1, wood, chemically activated, powder
Sigma-Aldrich
Activated Charcoal Norit®, Norit® GAC 1240W, from coal, for potable water processing, steam activated, granular
Sigma-Aldrich
Guanidine hydrochloride, for molecular biology, ≥99%
Sigma-Aldrich
Guanidine hydrochloride, ≥99% (titration), organic base and chaeotropic agent
Sigma-Aldrich
Guanidine hydrochloride, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
Guanidine hydrochloride, ≥99.0% (AT)
Sigma-Aldrich
Guanidine hydrochloride, ≥98%
Sigma-Aldrich
Carbon nanofibers, graphitized (iron-free), composed of conical platelets, D × L 100 nm × 20-200 μm
Sigma-Aldrich
Carbon nanofibers, graphitized, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm
Sigma-Aldrich
Guanidine hydrochloride, anhydrous, free-flowing, Redi-Dri, ≥99%
Carbon - Vitreous, rod, 200mm, diameter 1.0mm, glassy carbon
Carbon - Vitreous, foil, 100x100mm, thickness 2.0mm, glassy carbon
Carbon - Vitreous, foil, 8x8mm, thickness 0.5mm, glassy carbon
Carbon - Vitreous, foil, 100x100mm, thickness 1.0mm, glassy carbon
Carbon - Vitreous, rod, 200mm, diameter 10mm, glassy carbon
Carbon - Vitreous, foil, 100x100mm, thickness 6.0mm, glassy carbon