Merck
  • Home
  • Search Results
  • Self-assembled microbubbles as contrast agents for ultrasound/magnetic resonance dual-modality imaging.

Self-assembled microbubbles as contrast agents for ultrasound/magnetic resonance dual-modality imaging.

Acta biomaterialia (2015-06-27)
Sheng Song, Heze Guo, Zequan Jiang, Yuqing Jin, Ying Wu, Xiao An, Zhaofeng Zhang, Kang Sun, Hongjing Dou
ABSTRACT

In this work, superparamagnetic self-assembled microbubbles (SAMBs) consisting of "Poly(acrylic acid)-Iron oxide nanoparticles-Polyamine" sandwich-like shells and tetradecafluorohexane cores were fabricated by a template-free self-assembly approach. The SAMBs exhibit not only magnetic resonance (MR) T2 imaging functionality, but also ultrasound (US) image contrast, showing great potential as US/MR dual contrast agents. The diameters of the SAMBs can be tuned easily from 450nm to 1300nm by changing the precursor ratio, and this size variation directly affects their in vitro MRI and US signals. The SAMBs also exhibit in vivo contrast enhancement capabilities in rat liver with injection through portal vein, for both MR and US imaging. Additionally, the biodistribution of SAMBs over time suggests normal systemic metabolic activity through the spleen. The results show that the Fe content in rat liver reduces to a level of which Fe cannot be detected in 45days. The SAMBs exhibit no obvious damage to the primary organs of rat during the metabolic process, indicating their good biocompatibility in vivo.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Tetradecafluorohexane, A mixture of perfluorinated hexanes, 95%
Sigma-Aldrich
Phenylacetic acid, 99%
Sigma-Aldrich
Oleic acid, ≥99% (GC)
Sigma-Aldrich
S-Acetylthioglycolic acid N-hydroxysuccinimide ester, ≥95% (TLC), powder
Sigma-Aldrich
Phenylacetic acid, ≥99%, FCC, FG
Sigma-Aldrich
Oleic acid, natural, FCC
Sigma-Aldrich
Dimethyl sulfoxide, anhydrous, ≥99.9%
Sigma-Aldrich
N-Hydroxysuccinimide, 98%
Sigma-Aldrich
Oleic acid, technical grade, 90%
Sigma-Aldrich
Tetradecafluorohexane, 99%
Sigma-Aldrich
1-Octadecene, ≥95.0% (GC)
Sigma-Aldrich
Citric acid monohydrate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., buffer substance, 99.5-102%
Sigma-Aldrich
Sodium oleate, ≥82% (fatty acids), powder
Sigma-Aldrich
8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, suitable for fluorescence, ≥90% (HPCE)
Sigma-Aldrich
5α-Androstan-17β-ol-3-one, purum, ≥99.0% (TLC)
Sigma-Aldrich
1-Octadecene, technical grade, 90%
Sigma-Aldrich
Citric acid monohydrate, puriss., meets analytical specification of Ph. Eur., BP, USP, E330, 99.5-100.5% (based on anhydrous substance), grit
Sigma-Aldrich
Fluorescein sodium salt, BioReagent, suitable for fluorescence
Sigma-Aldrich
Fluorescein sodium salt, p.a., for source staining
Sigma-Aldrich
Oleic acid, meets analytical specification of Ph, Eur., 65.0-88.0% (GC)
Sigma-Aldrich
5α-Androstan-17β-ol-3-one, ≥97.5%
Sigma-Aldrich
Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
Fluorescein sodium salt, used as fluorescent tracer
Sigma-Aldrich
Oleic acid, suitable for cell culture, BioReagent
Sigma-Aldrich
Citric acid monohydrate, reagent grade, ≥98% (GC/titration)
Sigma-Aldrich
Citric acid monohydrate, BioXtra, ≥99.5%
Sigma-Aldrich
Citric acid monohydrate, ACS reagent, ≥99.0%
Sigma-Aldrich
Sodium oleate, ≥99%
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture