Looking for some tips and tricks on running immunoassays such as our MILLIPLEX® multiplex assays? Read on to get tips on reducing variability, correcting/preventing low bead counts, thorough plate washing, running antibody detection assays, immunoassay troubleshooting, and more.
Note: Protocol procedures are optimized for best data results. Consequently, protocols can vary from kit to kit. Therefore, it is important to read the entire protocol before running an assay. The protocol information described below is for 96-well plate formats unless otherwise indicated.
The key steps in the MILLIPLEX® assay protocol for the analysis of soluble biomarkers are:
Watch this quick video to see how to run MILLIPLEX® multiplex immunoassays.
Figure 1.Sample 96-well plate map showing placement of standards, QCs, and samples. In this format, 38 samples can be analyzed in duplicate. 384-well plate set-up is similar, but with 182 samples in duplicate.
If your samples are particularly sticky, it can help to resuspend the beads in 1X Wash Buffer before reading the plate on the instrument. The detergents in this buffer can help with any aggregation that may occur. Note that the plate must be read within four hours.
Figure 2.Example of a handheld magnetic separator block for 96-well plates.
Learn more about MILLIPLEX® Autoantibody Immunoassays in our “Multiplex Analysis of Autoantibodies” article.
Table 1 is a troubleshooting guide for running MILLIPLEX® multiplex assays.
Have more questions about MILLIPLEX® multiplex assays? Check out our FAQs or fill out the form by clicking on the button below.
For Research Use Only. Not For Use In Diagnostic Procedures.