Solid tumors grow in a three-dimensional (3D) spatial conformation, resulting in a heterogeneous exposure to oxygen and nutrients as well as to other physical and chemical stresses. To mimic the 3D spatial conformation, 3D in vitro culture models have been used in cancer research. Cancer stem cells (CSCs) are defined as a small subset of cells within a tumor with the ability to self-renew and often drive tumor progression and recurrence after chemotherapy treatment. Traditionally, cancer stem cells have been isolated from cancer cell lines and tumor biopsies and grown in 3D tumorsphere suspension cultures.
Figure 1.Overview of the Tumorsphere Formation Protocol
Figure 1. Tumorsphere formation of MCF7 Breast Cancer Cells at Passage 1 (A) and Passage 5 (B) cultured in 3dGRO™ Spheroid Medium.
Figure 2.Tumorsphere formation of E006AA Prostate Cancer Cells at Passage 1 (A) and Passage 5 (B) cultured in 3dGRO™ Spheroid Medium.